2e5l

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[[Image:2e5l.gif|left|200px]]
 
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==A snapshot of the 30S ribosomal subunit capturing mRNA via the Shine- Dalgarno interaction==
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The line below this paragraph, containing "STRUCTURE_2e5l", creates the "Structure Box" on the page.
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<StructureSection load='2e5l' size='340' side='right'caption='[[2e5l]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2e5l]] is a 11 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E5L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2E5L FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_2e5l| PDB=2e5l | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2e5l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2e5l OCA], [https://pdbe.org/2e5l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2e5l RCSB], [https://www.ebi.ac.uk/pdbsum/2e5l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2e5l ProSAT], [https://www.topsan.org/Proteins/RSGI/2e5l TOPSAN]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RS2_THET8 RS2_THET8] Spans the head-body hinge region of the 30S subunit. Is loosely associated with the 30S subunit.[HAMAP-Rule:MF_00291_B]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e5/2e5l_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2e5l ConSurf].
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<div style="clear:both"></div>
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'''A snapshot of the 30S ribosomal subunit capturing mRNA via the Shine- Dalgarno interaction'''
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==See Also==
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*[[Ribosomal protein THX 3D structures|Ribosomal protein THX 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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In the initiation phase of bacterial translation, the 30S ribosomal subunit captures mRNA in preparation for binding with initiator tRNA. The purine-rich Shine-Dalgarno (SD) sequence, in the 5' untranslated region of the mRNA, anchors the 30S subunit near the start codon, via base pairing with an anti-SD (aSD) sequence at the 3' terminus of 16S rRNA. Here, we present the 3.3 A crystal structure of the Thermus thermophilus 30S subunit bound with an mRNA mimic. The duplex formed by the SD and aSD sequences is snugly docked in a "chamber" between the head and platform domains, demonstrating how the 30S subunit captures and stabilizes the otherwise labile SD helix. This location of the SD helix is suitable for the placement of the start codon AUG in the immediate vicinity of the mRNA channel, in agreement with reported crosslinks between the second position of the start codon and G1530 of 16S rRNA.
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[[Category: Large Structures]]
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[[Category: Synthetic construct]]
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==About this Structure==
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2E5L is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E5L OCA].
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==Reference==
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A snapshot of the 30S ribosomal subunit capturing mRNA via the Shine-Dalgarno interaction., Kaminishi T, Wilson DN, Takemoto C, Harms JM, Kawazoe M, Schluenzen F, Hanawa-Suetsugu K, Shirouzu M, Fucini P, Yokoyama S, Structure. 2007 Mar;15(3):289-97. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17355865 17355865]
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[[Category: Protein complex]]
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[[Category: Thermus thermophilus]]
[[Category: Thermus thermophilus]]
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[[Category: Fucini, P.]]
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[[Category: Fucini P]]
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[[Category: Hanawa-Suetsugu, K.]]
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[[Category: Hanawa-Suetsugu K]]
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[[Category: Harms, J M.]]
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[[Category: Harms JM]]
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[[Category: Kaminishi, T.]]
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[[Category: Kaminishi T]]
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[[Category: Kawazoe, M.]]
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[[Category: Kawazoe M]]
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[[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]]
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[[Category: Schluenzen F]]
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[[Category: Schluenzen, F.]]
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[[Category: Shirouzu M]]
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[[Category: Shirouzu, M.]]
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[[Category: Takemoto C]]
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[[Category: Takemoto, C.]]
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[[Category: Wilson DN]]
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[[Category: Wilson, D N.]]
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[[Category: Yokoyama S]]
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[[Category: Yokoyama, S.]]
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[[Category: 30s ribosomal subunit]]
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[[Category: 5' untranslated region]]
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[[Category: Mrna capture]]
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[[Category: National project on protein structural and functional analyse]]
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[[Category: Nppsfa]]
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[[Category: Riken structural genomics/proteomics initiative]]
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[[Category: Rsgi]]
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[[Category: Shine-dalgarno interaction]]
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[[Category: Structural genomic]]
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[[Category: Translation initiation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 01:57:21 2008''
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Current revision

A snapshot of the 30S ribosomal subunit capturing mRNA via the Shine- Dalgarno interaction

PDB ID 2e5l

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