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2hpa

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(New page: 200px<br /> <applet load="2hpa" size="450" color="white" frame="true" align="right" spinBox="true" caption="2hpa, resolution 2.90&Aring;" /> '''STRUCTURAL ORIGINS ...)
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[[Image:2hpa.gif|left|200px]]<br />
 
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<applet load="2hpa" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2hpa, resolution 2.90&Aring;" />
 
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'''STRUCTURAL ORIGINS OF L(+)-TARTRATE INHIBITION OF HUMAN PROSTATIC ACID PHOSPHATASE'''<br />
 
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==Overview==
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==STRUCTURAL ORIGINS OF L(+)-TARTRATE INHIBITION OF HUMAN PROSTATIC ACID PHOSPHATASE==
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Acid phosphatase activity in the blood serum is usually separated into, tartrate-resistant and tartrate-refractory, which is reported as the, prostatic acid phosphatase level. Human prostatic acid phosphatase, crystals soaked in N-propyl-L-tartramate were used to collect x-ray, diffraction data to 2.9 A resolution under cryogenic conditions. Positive, difference electron density, corresponding to the inhibitor, was found., The quality of the electron density maps clearly shows the orientation of, the carboxylate and N-propyl-substituted amide groups. The hydroxyl group, attached to C3 forms two crucial hydrogen bonds with Arg-79 and His-257., Previous crystallographic studies compiled on the tartrate-rat prostatic, acid phosphatase binary complex (Lindqvist, Y., Schneider, G., and Vihko, P. (1993) J. Biol. Chem. 268, 20744-20746) erroneously positioned, D-tartrate into the active site. Modeling studies have shown that the C3, hydroxyl group on the D(-)-stereoisomer of tartrate, which does not, significantly inhibit prostatic acid phosphatase, does not form strong, hydrogen bonds with Arg-79 or His-257. The structure of human prostatic, acid phosphatase, noncovalently bound in N-propyl-L-tartramate, is used to, develop inhibitors with higher specificity and potency than L(+)-tartrate.
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<StructureSection load='2hpa' size='340' side='right'caption='[[2hpa]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2hpa]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HPA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HPA FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PT3:N-PROPYL-TARTRAMIC+ACID'>PT3</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Acid_phosphatase Acid phosphatase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hpa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hpa OCA], [https://pdbe.org/2hpa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hpa RCSB], [https://www.ebi.ac.uk/pdbsum/2hpa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hpa ProSAT]</span></td></tr>
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</table>
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== Function ==
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[[https://www.uniprot.org/uniprot/PPAP_HUMAN PPAP_HUMAN]] A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma.<ref>PMID:15280042</ref> <ref>PMID:18083097</ref> <ref>PMID:19403677</ref> <ref>PMID:20498373</ref> Isoform 2: the cellular form also has ecto-5'-nucleotidase activity in dorsal root ganglion (DRG) neurons. Generates adenosine from AMP which acts as a pain suppressor. Acts as a tumor suppressor of prostate cancer through dephosphorylation of ERBB2 and deactivation of MAPK-mediated signaling.<ref>PMID:15280042</ref> <ref>PMID:18083097</ref> <ref>PMID:19403677</ref> <ref>PMID:20498373</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hp/2hpa_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hpa ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Acid phosphatase activity in the blood serum is usually separated into tartrate-resistant and tartrate-refractory, which is reported as the prostatic acid phosphatase level. Human prostatic acid phosphatase crystals soaked in N-propyl-L-tartramate were used to collect x-ray diffraction data to 2.9 A resolution under cryogenic conditions. Positive difference electron density, corresponding to the inhibitor, was found. The quality of the electron density maps clearly shows the orientation of the carboxylate and N-propyl-substituted amide groups. The hydroxyl group attached to C3 forms two crucial hydrogen bonds with Arg-79 and His-257. Previous crystallographic studies compiled on the tartrate-rat prostatic acid phosphatase binary complex (Lindqvist, Y., Schneider, G., and Vihko, P. (1993) J. Biol. Chem. 268, 20744-20746) erroneously positioned D-tartrate into the active site. Modeling studies have shown that the C3 hydroxyl group on the D(-)-stereoisomer of tartrate, which does not significantly inhibit prostatic acid phosphatase, does not form strong hydrogen bonds with Arg-79 or His-257. The structure of human prostatic acid phosphatase, noncovalently bound in N-propyl-L-tartramate, is used to develop inhibitors with higher specificity and potency than L(+)-tartrate.
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==About this Structure==
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Structural origins of L(+)-tartrate inhibition of human prostatic acid phosphatase.,LaCount MW, Handy G, Lebioda L J Biol Chem. 1998 Nov 13;273(46):30406-9. PMID:9804805<ref>PMID:9804805</ref>
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2HPA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with NAG, NDG and PT3 as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Acid_phosphatase Acid phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2HPA OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structural origins of L(+)-tartrate inhibition of human prostatic acid phosphatase., LaCount MW, Handy G, Lebioda L, J Biol Chem. 1998 Nov 13;273(46):30406-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9804805 9804805]
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</div>
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<div class="pdbe-citations 2hpa" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Acid phosphatase 3D structures|Acid phosphatase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Acid phosphatase]]
[[Category: Acid phosphatase]]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Handy, G.]]
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[[Category: Handy, G]]
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[[Category: Lacount, M.W.]]
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[[Category: Lacount, M W]]
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[[Category: Lebioda, L.]]
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[[Category: Lebioda, L]]
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[[Category: NAG]]
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[[Category: Hydrolase]]
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[[Category: NDG]]
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[[Category: N-propyltartramate]]
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[[Category: PT3]]
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[[Category: acid phosphatase]]
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[[Category: n-propyltartramate]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 22:34:51 2007''
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Current revision

STRUCTURAL ORIGINS OF L(+)-TARTRATE INHIBITION OF HUMAN PROSTATIC ACID PHOSPHATASE

PDB ID 2hpa

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