2ie4

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /> <applet load="2ie4" size="450" color="white" frame="true" align="right" spinBox="true" caption="2ie4, resolution 2.6&Aring;" /> '''Structure of the Pro...)
Current revision (09:02, 21 February 2024) (edit) (undo)
 
(15 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2ie4.gif|left|200px]]<br />
 
-
<applet load="2ie4" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="2ie4, resolution 2.6&Aring;" />
 
-
'''Structure of the Protein Phosphatase 2A Core Enzyme Bound to okadaic acid'''<br />
 
-
==Overview==
+
==Structure of the Protein Phosphatase 2A Core Enzyme Bound to okadaic acid==
-
The serine/threonine phosphatase protein phosphatase 2A (PP2A) plays an, essential role in many aspects of cellular functions and has been shown to, be an important tumor suppressor. The core enzyme of PP2A comprises a 65, kDa scaffolding subunit and a 36 kDa catalytic subunit. Here we report the, crystal structures of the PP2A core enzyme bound to two of its inhibitors, the tumor-inducing agents okadaic acid and microcystin-LR, at 2.6 and 2.8, A resolution, respectively. The catalytic subunit recognizes one end of, the elongated scaffolding subunit by interacting with the conserved ridges, of HEAT repeats 11-15. Formation of the core enzyme forces the scaffolding, subunit to undergo pronounced structural rearrangement. The scaffolding, subunit exhibits considerable conformational flexibility, which is, proposed to play an essential role in PP2A function. These structures, together with biochemical analyses, reveal significant insights into PP2A, function and serve as a framework for deciphering the diverse roles of, PP2A in cellular physiology.
+
<StructureSection load='2ie4' size='340' side='right'caption='[[2ie4]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2ie4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IE4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IE4 FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=OKA:OKADAIC+ACID'>OKA</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ie4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ie4 OCA], [https://pdbe.org/2ie4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ie4 RCSB], [https://www.ebi.ac.uk/pdbsum/2ie4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ie4 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/2AAA_HUMAN 2AAA_HUMAN] The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Required for proper chromosome segregation and for centromeric localization of SGOL1 in mitosis.<ref>PMID:16580887</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ie/2ie4_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ie4 ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
2IE4 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with MN and OKA as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2IE4 OCA].
+
*[[Protein phosphatase 3D structures|Protein phosphatase 3D structures]]
-
 
+
== References ==
-
==Reference==
+
<references/>
-
Structure of protein phosphatase 2A core enzyme bound to tumor-inducing toxins., Xing Y, Xu Y, Chen Y, Jeffrey PD, Chao Y, Lin Z, Li Z, Strack S, Stock JB, Shi Y, Cell. 2006 Oct 20;127(2):341-53. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17055435 17055435]
+
__TOC__
 +
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Phosphoprotein phosphatase]]
+
[[Category: Large Structures]]
-
[[Category: Protein complex]]
+
[[Category: Chao Y]]
-
[[Category: Chao, Y.]]
+
[[Category: Chen Y]]
-
[[Category: Chen, Y.]]
+
[[Category: Jeffrey PD]]
-
[[Category: Jeffrey, P.D.]]
+
[[Category: Shi Y]]
-
[[Category: Shi, Y.]]
+
[[Category: Xing Y]]
-
[[Category: Xing, Y.]]
+
[[Category: Xu Y]]
-
[[Category: Xu, Y.]]
+
-
[[Category: MN]]
+
-
[[Category: OKA]]
+
-
[[Category: heat repeat]]
+
-
[[Category: protein-protein complex]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 22:43:54 2007''
+

Current revision

Structure of the Protein Phosphatase 2A Core Enzyme Bound to okadaic acid

PDB ID 2ie4

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ie4"
Personal tools