2eul

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[[Image:2eul.gif|left|200px]]
 
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==Structure of the transcription factor Gfh1.==
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The line below this paragraph, containing "STRUCTURE_2eul", creates the "Structure Box" on the page.
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<StructureSection load='2eul' size='340' side='right'caption='[[2eul]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2eul]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EUL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EUL FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_2eul| PDB=2eul | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2eul FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eul OCA], [https://pdbe.org/2eul PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2eul RCSB], [https://www.ebi.ac.uk/pdbsum/2eul PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2eul ProSAT], [https://www.topsan.org/Proteins/RSGI/2eul TOPSAN]</span></td></tr>
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</table>
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'''Structure of the transcription factor Gfh1.'''
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== Function ==
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[https://www.uniprot.org/uniprot/GFH1_THET8 GFH1_THET8] Inhibits all catalytic activities of RNA polymerase (RNAP) by partially occluding its substrate-binding site and preventing NTP binding.<ref>PMID:11606592</ref> <ref>PMID:14712703</ref> <ref>PMID:16298991</ref>
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Gre factors enhance the intrinsic endonucleolytic activity of RNA polymerase to rescue arrested transcription complexes and are thought to confer the high fidelity and processivity of RNA synthesis. The Gre factors insert the extended alpha-helical coiled-coil domains into the RNA polymerase secondary channel to position two invariant acidic residues at the coiled-coil tip near the active site to stabilize the catalytic metal ion. Gfh1, a GreA homolog from Thermus thermophilus, inhibits rather than activates RNA cleavage. Here we report the structure of the T. thermophilus Gfh1 at 2.4 A resolution revealing a two-domain architecture closely resembling that of GreA. However, the interdomain orientation is strikingly distinct (approximately 162 degrees rotation) between the two proteins. In contrast to GreA, which has two acidic residues on a well fixed self-stabilized alpha-turn, the tip of the Gfh1 coiled-coil is flexible and contains four acidic residues. This difference is likely the key to the Gre functional diversity, while Gfh1 inhibits exo- and endonucleolytic cleavage, RNA synthesis, and pyrophosphorolysis, GreA enhances only the endonucleolytic cleavage. We propose that Gfh1 acidic residues stabilize the RNA polymerase active center in a catalytically inactive configuration through Mg2+-mediated interactions. The excess of the acidic residues and inherent flexibility of the coiled-coil tip might allow Gfh1 to adjust its activity to structurally distinct substrates, thereby inhibiting diverse catalytic reactions of RNA polymerase.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eu/2eul_consurf.spt"</scriptWhenChecked>
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2EUL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EUL OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Regulation through the RNA polymerase secondary channel. Structural and functional variability of the coiled-coil transcription factors., Symersky J, Perederina A, Vassylyeva MN, Svetlov V, Artsimovitch I, Vassylyev DG, J Biol Chem. 2006 Jan 20;281(3):1309-12. Epub 2005 Nov 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16298991 16298991]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2eul ConSurf].
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[[Category: Single protein]]
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<div style="clear:both"></div>
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[[Category: Thermus thermophilus]]
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== References ==
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[[Category: Artsimovitch, I.]]
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<references/>
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[[Category: Perederina, A.]]
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__TOC__
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[[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]]
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</StructureSection>
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[[Category: Svetlov, V.]]
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[[Category: Large Structures]]
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[[Category: Symersky, J.]]
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[[Category: Thermus thermophilus HB8]]
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[[Category: Vassylyev, D G.]]
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[[Category: Artsimovitch I]]
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[[Category: Vassylyeva, M N.]]
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[[Category: Perederina A]]
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[[Category: Gfh1]]
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[[Category: Svetlov V]]
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[[Category: Riken structural genomics/proteomics initiative]]
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[[Category: Symersky J]]
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[[Category: Rna polymerase]]
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[[Category: Vassylyev DG]]
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[[Category: Rsgi]]
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[[Category: Vassylyeva MN]]
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[[Category: Structural genomic]]
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[[Category: Transcription factor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:07:52 2008''
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Current revision

Structure of the transcription factor Gfh1.

PDB ID 2eul

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