2f1f

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[[Image:2f1f.gif|left|200px]]
 
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==Crystal structure of the regulatory subunit of acetohydroxyacid synthase isozyme III from E. coli==
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The line below this paragraph, containing "STRUCTURE_2f1f", creates the "Structure Box" on the page.
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<StructureSection load='2f1f' size='340' side='right'caption='[[2f1f]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2f1f]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F1F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2F1F FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=P33:3,6,9,12,15,18-HEXAOXAICOSANE-1,20-DIOL'>P33</scene></td></tr>
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{{STRUCTURE_2f1f| PDB=2f1f | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2f1f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f1f OCA], [https://pdbe.org/2f1f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2f1f RCSB], [https://www.ebi.ac.uk/pdbsum/2f1f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2f1f ProSAT]</span></td></tr>
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</table>
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'''Crystal structure of the regulatory subunit of acetohydroxyacid synthase isozyme III from E. coli'''
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== Function ==
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[https://www.uniprot.org/uniprot/ILVH_ECOLI ILVH_ECOLI]
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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The enzyme acetohydroxyacid synthase (AHAS) catalyses the first common step in the biosynthesis of the three branched-chain amino acids. Enzymes in the AHAS family generally consist of regulatory and catalytic subunits. Here, we describe the first crystal structure of an AHAS regulatory subunit, the ilvH polypeptide, determined at a resolution of 1.75 A. IlvH is the regulatory subunit of one of three AHAS isozymes expressed in Escherichia coli, AHAS III. The protein is a dimer, with two beta alpha beta beta alpha beta ferredoxin domains in each monomer. The two N-terminal domains assemble to form an ACT domain structure remarkably close to the one predicted by us on the basis of the regulatory domain of 3-phosphoglycerate dehydrogenase (3PGDH). The two C-terminal domains combine so that their beta-sheets are roughly positioned back-to-back and perpendicular to the extended beta-sheet of the N-terminal ACT domain. On the basis of the properties of mutants and a comparison with 3PGDH, the effector (valine) binding sites can be located tentatively in two symmetrically related positions in the interface between a pair of N-terminal domains. The properties of mutants of the ilvH polypeptide outside the putative effector-binding site provide further insight into the functioning of the holoenzyme. The results of this study open avenues for further studies aimed at understanding the mechanism of regulation of AHAS by small-molecule effectors.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f1/2f1f_consurf.spt"</scriptWhenChecked>
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2F1F is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F1F OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Structure of the regulatory subunit of acetohydroxyacid synthase isozyme III from Escherichia coli., Kaplun A, Vyazmensky M, Zherdev Y, Belenky I, Slutzker A, Mendel S, Barak Z, Chipman DM, Shaanan B, J Mol Biol. 2006 Mar 31;357(3):951-63. Epub 2006 Jan 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16458324 16458324]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2f1f ConSurf].
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[[Category: Acetolactate synthase]]
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Barak, Z.]]
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[[Category: Barak Z]]
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[[Category: Chipman, D M.]]
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[[Category: Chipman DM]]
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[[Category: Kaplun, A.]]
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[[Category: Kaplun A]]
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[[Category: Shaanan, B.]]
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[[Category: Shaanan B]]
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[[Category: Vyazmensky, M.]]
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[[Category: Vyazmensky M]]
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[[Category: Act domain]]
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[[Category: Ferredoxin fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:20:57 2008''
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Current revision

Crystal structure of the regulatory subunit of acetohydroxyacid synthase isozyme III from E. coli

PDB ID 2f1f

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