This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2f31

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:21, 14 February 2024) (edit) (undo)
 
(10 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2f31.gif|left|200px]]
 
-
<!--
+
==Crystal structure of the autoinhibitory switch in Formin mDia1; the DID/DAD complex==
-
The line below this paragraph, containing "STRUCTURE_2f31", creates the "Structure Box" on the page.
+
<StructureSection load='2f31' size='340' side='right'caption='[[2f31]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2f31]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F31 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2F31 FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
-
-->
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2f31 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f31 OCA], [https://pdbe.org/2f31 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2f31 RCSB], [https://www.ebi.ac.uk/pdbsum/2f31 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2f31 ProSAT]</span></td></tr>
-
{{STRUCTURE_2f31| PDB=2f31 | SCENE= }}
+
</table>
-
 
+
== Function ==
-
'''Crystal structure of the autoinhibitory switch in Formin mDia1; the DID/DAD complex'''
+
[https://www.uniprot.org/uniprot/Q3US76_MOUSE Q3US76_MOUSE]
-
 
+
== Evolutionary Conservation ==
-
 
+
[[Image:Consurf_key_small.gif|200px|right]]
-
==Overview==
+
Check<jmol>
-
Diaphanous-related formins (DRFs) regulate the nucleation and polymerization of unbranched actin filaments. The activity of DRFs is inhibited by an intramolecular interaction between their N-terminal regulatory region and a conserved C-terminal segment termed the Diaphanous autoinhibitory domain (DAD). Binding of GTP bound Rho to the mDia1 N terminus releases this autoinhibitory restraint. Here, we describe the crystal structure of the DAD segment of mDia1 in complex with the relevant N-terminal fragment, termed the DID domain. The structure reveals that the DAD segment forms an amphipathic helix that binds a conserved, concave surface on the DID domain. Comparison with the structure of the mDia1 N terminus bound to RhoC suggests that release of the autoinhibitory DAD interaction is accomplished largely by Rho-induced restructuring of the adjacent GTPase binding subdomain (GBD), but also by electrostatic repulsion and a small, direct steric occlusion of the DAD binding cleft by Rho itself.
+
<jmolCheckbox>
-
 
+
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f3/2f31_consurf.spt"</scriptWhenChecked>
-
==About this Structure==
+
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-
2F31 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F31 OCA].
+
<text>to colour the structure by Evolutionary Conservation</text>
-
 
+
</jmolCheckbox>
-
==Reference==
+
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2f31 ConSurf].
-
Structure of the autoinhibitory switch in formin mDia1., Nezami AG, Poy F, Eck MJ, Structure. 2006 Feb;14(2):257-63. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16472745 16472745]
+
<div style="clear:both"></div>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
-
[[Category: Protein complex]]
+
[[Category: Eck MJ]]
-
[[Category: Eck, M J.]]
+
[[Category: Nezami AG]]
-
[[Category: Nezami, A G.]]
+
[[Category: Poy F]]
-
[[Category: Poy, F.]]
+
-
[[Category: Armadillo repeat]]
+
-
[[Category: Formin,mdia1]]
+
-
[[Category: Protein-protein complex]]
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:24:25 2008''
+

Current revision

Crystal structure of the autoinhibitory switch in Formin mDia1; the DID/DAD complex

PDB ID 2f31

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2f31"
Personal tools