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| - | [[Image:2f40.gif|left|200px]] | |
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| - | <!--
| + | ==Structure of a Novel Protein from Backbone-Centered NMR Data and NMR-Assisted Structure Prediction== |
| - | The line below this paragraph, containing "STRUCTURE_2f40", creates the "Structure Box" on the page.
| + | <StructureSection load='2f40' size='340' side='right'caption='[[2f40]]' scene=''> |
| - | You may change the PDB parameter (which sets the PDB file loaded into the applet)
| + | == Structural highlights == |
| - | or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| + | <table><tr><td colspan='2'>[[2f40]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus_DSM_3638 Pyrococcus furiosus DSM 3638]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F40 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2F40 FirstGlance]. <br> |
| - | or leave the SCENE parameter empty for the default display.
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | -->
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2f40 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f40 OCA], [https://pdbe.org/2f40 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2f40 RCSB], [https://www.ebi.ac.uk/pdbsum/2f40 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2f40 ProSAT]</span></td></tr> |
| - | {{STRUCTURE_2f40| PDB=2f40 | SCENE= }}
| + | </table> |
| - | | + | == Function == |
| - | '''Structure of a Novel Protein from Backbone-Centered NMR Data and NMR-Assisted Structure Prediction'''
| + | [https://www.uniprot.org/uniprot/Q8U0X6_PYRFU Q8U0X6_PYRFU] |
| - | | + | __TOC__ |
| - | | + | </StructureSection> |
| - | ==Overview== | + | [[Category: Large Structures]] |
| - | Targeting of proteins for structure determination in structural genomic programs often includes the use of threading and fold recognition methods to exclude proteins belonging to well-populated fold families, but such methods can still fail to recognize preexisting folds. The authors illustrate here a method in which limited amounts of structural data are used to improve an initial homology search and the data are subsequently used to produce a structure by data-constrained refinement of an identified structural template. The data used are primarily NMR-based residual dipolar couplings, but they also include additional chemical shift and backbone-nuclear Overhauser effect data. Using this methodology, a backbone structure was efficiently produced for a 10 kDa protein (PF1455) from Pyrococcus furiosus. Its relationship to existing structures and its probable function are discussed.
| + | [[Category: Pyrococcus furiosus DSM 3638]] |
| - | | + | [[Category: Bansal S]] |
| - | ==About this Structure== | + | [[Category: Prestegard JH]] |
| - | 2F40 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F40 OCA].
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| - | | + | |
| - | ==Reference==
| + | |
| - | Structure determination of a new protein from backbone-centered NMR data and NMR-assisted structure prediction., Mayer KL, Qu Y, Bansal S, LeBlond PD, Jenney FE Jr, Brereton PS, Adams MW, Xu Y, Prestegard JH, Proteins. 2006 Nov 1;65(2):480-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16927360 16927360]
| + | |
| - | [[Category: Pyrococcus furiosus]] | + | |
| - | [[Category: Single protein]]
| + | |
| - | [[Category: Bansal, S.]] | + | |
| - | [[Category: Prestegard, J H.]] | + | |
| - | [[Category: SECSG, Southeast Collaboratory for Structural Genomics.]] | + | |
| - | [[Category: Protein structure initiative]] | + | |
| - | [[Category: Protein structure prediction]]
| + | |
| - | [[Category: Psi]]
| + | |
| - | [[Category: Pyrococcus furious]]
| + | |
| - | [[Category: Residual dipolar coupling]] | + | |
| - | [[Category: Secsg]] | + | |
| - | [[Category: Simulated annealing]] | + | |
| - | [[Category: Southeast collaboratory for structural genomic]] | + | |
| - | [[Category: Structural genomic]] | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:26:16 2008''
| + | |
