2f4f

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[[Image:2f4f.gif|left|200px]]
 
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==Crystal structure of IS200 transposase==
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The line below this paragraph, containing "STRUCTURE_2f4f", creates the "Structure Box" on the page.
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<StructureSection load='2f4f' size='340' side='right'caption='[[2f4f]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2f4f]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus Saccharolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F4F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2F4F FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
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{{STRUCTURE_2f4f| PDB=2f4f | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2f4f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f4f OCA], [https://pdbe.org/2f4f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2f4f RCSB], [https://www.ebi.ac.uk/pdbsum/2f4f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2f4f ProSAT]</span></td></tr>
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</table>
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'''Crystal structure of IS200 transposase'''
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== Function ==
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[https://www.uniprot.org/uniprot/Q97Y68_SACS2 Q97Y68_SACS2]
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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IS200 transposases, present in many bacteria and Archaea, appear to be distinct from other groups of transposases. To provide a structural basis for understanding the action of IS200 transposases, we have determined the crystal structure of the SSO1474 protein from Sulfolobus solfataricus, a member of the IS200 family, in both Mn(2+)-bound and Mn(2+)-free forms. Its monomer fold is distinct from other classes of structurally characterized transposases. Two monomers form a tight dimer by exchanging the C-terminal alpha-helix and by merging the two central beta-sheets into a large beta-sheet. Glu(55), His(62), and four water molecules provide the direct coordination sphere of the catalytically essential metal ion in the Mn(2+)-bound structure. His(16), Asp(59), and His(60) also play important roles in maintaining the metal binding site. The catalytic site is formed at the interface between monomers. The candidate nucleophile in the transposition mechanism, strictly conserved Tyr(121) coming from the other monomer, is turned away from the active site, suggesting that a conformational change is likely to occur during the catalytic cycle.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f4/2f4f_consurf.spt"</scriptWhenChecked>
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2F4F is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F4F OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Crystal structure of a metal ion-bound IS200 transposase., Lee HH, Yoon JY, Kim HS, Kang JY, Kim KH, Kim DJ, Ha JY, Mikami B, Yoon HJ, Suh SW, J Biol Chem. 2006 Feb 17;281(7):4261-6. Epub 2005 Dec 11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16340015 16340015]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2f4f ConSurf].
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[[Category: Single protein]]
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<div style="clear:both"></div>
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[[Category: Sulfolobus solfataricus]]
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__TOC__
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[[Category: Kang, J Y.]]
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</StructureSection>
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[[Category: Kim, D J.]]
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[[Category: Large Structures]]
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[[Category: Kim, H S.]]
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[[Category: Saccharolobus solfataricus]]
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[[Category: Kim, K H.]]
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[[Category: Kang JY]]
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[[Category: Lee, H H.]]
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[[Category: Kim DJ]]
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[[Category: Suh, S W.]]
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[[Category: Kim HS]]
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[[Category: Yoon, J Y.]]
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[[Category: Kim KH]]
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[[Category: Mn complex]]
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[[Category: Lee HH]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:26:54 2008''
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[[Category: Suh SW]]
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[[Category: Yoon JY]]

Current revision

Crystal structure of IS200 transposase

PDB ID 2f4f

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