2f9t

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[[Image:2f9t.gif|left|200px]]
 
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==Structure of the type III CoaA from Pseudomonas aeruginosa==
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The line below this paragraph, containing "STRUCTURE_2f9t", creates the "Structure Box" on the page.
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<StructureSection load='2f9t' size='340' side='right'caption='[[2f9t]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2f9t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F9T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2F9T FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_2f9t| PDB=2f9t | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2f9t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f9t OCA], [https://pdbe.org/2f9t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2f9t RCSB], [https://www.ebi.ac.uk/pdbsum/2f9t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2f9t ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/COAX_PSEAE COAX_PSEAE] Catalyzes the phosphorylation of pantothenate (Pan), the first step in CoA biosynthesis. Can utilize a wide range of phosphoryl donors other than ATP, and does not discriminate between purine- and pyrimidine-based nucleotides or deoxynucleotides. Is responsible for the resistance of P.aeruginosa to the pantothenamide antibiotics, since it can not bind and phosphorylate these pantothenate analogs.<ref>PMID:16905099</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f9/2f9t_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2f9t ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Three distinct isoforms of pantothenate kinase (CoaA) in bacteria catalyze the first step in coenzyme A biosynthesis. The structures of the type II (Staphylococcus aureus, SaCoaA) and type III (Pseudomonas aeruginosa, PaCoaA) enzymes reveal that they assemble nearly identical subunits with actin-like folds into dimers that exhibit distinct biochemical properties. PaCoaA has a fully enclosed pantothenate binding pocket and requires a monovalent cation to weakly bind ATP in an open cavity that does not interact with the adenine nucleotide. Pantothenate binds to an open pocket in SaCoaA that strongly binds ATP by using a classical P loop architecture coupled with specific interactions with the adenine moiety. The PaCoaA*Pan binary complex explains the resistance of bacteria possessing this isoform to the pantothenamide antibiotics, and the similarity between SaCoaA and human pantothenate kinase 2 explains the molecular basis for the development of the neurodegenerative phenotype in three mutations in the human protein.
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'''Structure of the type III CoaA from Pseudomonas aeruginosa'''
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Prokaryotic type II and type III pantothenate kinases: The same monomer fold creates dimers with distinct catalytic properties.,Hong BS, Yun MK, Zhang YM, Chohnan S, Rock CO, White SW, Jackowski S, Park HW, Leonardi R Structure. 2006 Aug;14(8):1251-61. PMID:16905099<ref>PMID:16905099</ref>
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==Overview==
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Three distinct isoforms of pantothenate kinase (CoaA) in bacteria catalyze the first step in coenzyme A biosynthesis. The structures of the type II (Staphylococcus aureus, SaCoaA) and type III (Pseudomonas aeruginosa, PaCoaA) enzymes reveal that they assemble nearly identical subunits with actin-like folds into dimers that exhibit distinct biochemical properties. PaCoaA has a fully enclosed pantothenate binding pocket and requires a monovalent cation to weakly bind ATP in an open cavity that does not interact with the adenine nucleotide. Pantothenate binds to an open pocket in SaCoaA that strongly binds ATP by using a classical P loop architecture coupled with specific interactions with the adenine moiety. The PaCoaA*Pan binary complex explains the resistance of bacteria possessing this isoform to the pantothenamide antibiotics, and the similarity between SaCoaA and human pantothenate kinase 2 explains the molecular basis for the development of the neurodegenerative phenotype in three mutations in the human protein.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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2F9T is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F9T OCA].
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</div>
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<div class="pdbe-citations 2f9t" style="background-color:#fffaf0;"></div>
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==Reference==
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==See Also==
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Prokaryotic type II and type III pantothenate kinases: The same monomer fold creates dimers with distinct catalytic properties., Hong BS, Yun MK, Zhang YM, Chohnan S, Rock CO, White SW, Jackowski S, Park HW, Leonardi R, Structure. 2006 Aug;14(8):1251-61. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16905099 16905099]
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*[[Pantothenate kinase 3D structures|Pantothenate kinase 3D structures]]
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[[Category: Pantothenate kinase]]
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== References ==
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[[Category: Pseudomonas aeruginosa pao1]]
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<references/>
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[[Category: Single protein]]
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__TOC__
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[[Category: Chohnan, S.]]
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</StructureSection>
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[[Category: Jackowski, S.]]
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[[Category: Large Structures]]
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[[Category: Leonardi, R.]]
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[[Category: Pseudomonas aeruginosa PAO1]]
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[[Category: Rock, C O.]]
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[[Category: Chohnan S]]
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[[Category: White, S W.]]
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[[Category: Jackowski S]]
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[[Category: Yun, M K.]]
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[[Category: Leonardi R]]
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[[Category: Coaa]]
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[[Category: Rock CO]]
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[[Category: Pantothenate kinase]]
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[[Category: White SW]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:38:28 2008''
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[[Category: Yun MK]]

Current revision

Structure of the type III CoaA from Pseudomonas aeruginosa

PDB ID 2f9t

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