2fm8

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[[Image:2fm8.gif|left|200px]]
 
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==Crystal Structure of the Salmonella Secretion Chaperone InvB in Complex with SipA==
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The line below this paragraph, containing "STRUCTURE_2fm8", creates the "Structure Box" on the page.
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<StructureSection load='2fm8' size='340' side='right'caption='[[2fm8]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2fm8]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FM8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FM8 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fm8 OCA], [https://pdbe.org/2fm8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fm8 RCSB], [https://www.ebi.ac.uk/pdbsum/2fm8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fm8 ProSAT]</span></td></tr>
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{{STRUCTURE_2fm8| PDB=2fm8 | SCENE= }}
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</table>
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== Function ==
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'''Crystal Structure of the Salmonella Secretion Chaperone InvB in Complex with SipA'''
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[https://www.uniprot.org/uniprot/SPAK_SALTY SPAK_SALTY] Involved in a secretory pathway responsible for the surface presentation of determinants needed for the entry of Salmonella species into mammalian cells. Chaperone specialized in the storage of effectors within the bacterial cytoplasm, maintaining them in a secretion-competent state, and allowing their immediate delivery to target cells upon contact of the bacterium with the host cells. Has been shown to chaperone SopA, SopE, SopE2 and SipA.<ref>PMID:16547027</ref> <ref>PMID:16507363</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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Salmonella invasion protein A (SipA) is translocated into host cells by a type III secretion system (T3SS) and comprises two regions: one domain binds its cognate type III secretion chaperone, InvB, in the bacterium to facilitate translocation, while a second domain functions in the host cell, contributing to bacterial uptake by polymerizing actin. We present here the crystal structures of the SipA chaperone binding domain (CBD) alone and in complex with InvB. The SipA CBD is found to consist of a nonglobular polypeptide as well as a large globular domain, both of which are necessary for binding to InvB. We also identify a structural motif that may direct virulence factors to their cognate chaperones in a diverse range of pathogenic bacteria. Disruption of this structural motif leads to a destabilization of several chaperone-substrate complexes from different species, as well as an impairment of secretion in Salmonella.
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fm/2fm8_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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2FM8 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FM8 OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fm8 ConSurf].
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A common structural motif in the binding of virulence factors to bacterial secretion chaperones., Lilic M, Vujanac M, Stebbins CE, Mol Cell. 2006 Mar 3;21(5):653-64. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16507363 16507363]
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<div style="clear:both"></div>
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[[Category: Protein complex]]
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== References ==
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[[Category: Salmonella typhimurium]]
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<references/>
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[[Category: Lilic, M.]]
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__TOC__
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[[Category: Stebbins, C E.]]
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</StructureSection>
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[[Category: Vujanac, M.]]
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[[Category: Large Structures]]
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[[Category: Bacterial]]
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[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
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[[Category: Chaperone]]
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[[Category: Lilic M]]
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[[Category: Protein folding]]
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[[Category: Stebbins CE]]
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[[Category: Salmonella]]
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[[Category: Vujanac M]]
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[[Category: Translocation]]
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[[Category: Type iii secretion]]
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[[Category: Virulence]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:03:43 2008''
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Current revision

Crystal Structure of the Salmonella Secretion Chaperone InvB in Complex with SipA

PDB ID 2fm8

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