2fsj

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[[Image:2fsj.gif|left|200px]]
 
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==Crystal structure of Ta0583, an archaeal actin homolog, native data==
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The line below this paragraph, containing "STRUCTURE_2fsj", creates the "Structure Box" on the page.
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<StructureSection load='2fsj' size='340' side='right'caption='[[2fsj]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2fsj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermoplasma_acidophilum_DSM_1728 Thermoplasma acidophilum DSM 1728]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FSJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FSJ FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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{{STRUCTURE_2fsj| PDB=2fsj | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fsj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fsj OCA], [https://pdbe.org/2fsj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fsj RCSB], [https://www.ebi.ac.uk/pdbsum/2fsj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fsj ProSAT]</span></td></tr>
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</table>
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'''Crystal structure of Ta0583, an archaeal actin homolog, native data'''
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== Function ==
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[https://www.uniprot.org/uniprot/ACTH_THEAC ACTH_THEAC] Polymerizes into bundles of filaments, forming a helix with a filament width of 5.5 nm and an axial repeating unit of 5.5 nm. Polymerization of Ta0583 requires NTP and is optimal with ATP, but GTP, UTP, CTP, and even the deoxy form of NTP can also support the polymerization reaction. Nucleoside diphosphate or AMP-PNP does not support polymerization.<ref>PMID:16500678</ref> <ref>PMID:17189356</ref>
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Prokaryotic homologs of the eukaryotic structural protein actin, such as MreB and ParM, have been implicated in determination of bacterial cell shape, and in the segregation of genomic and plasmid DNA. In contrast to these bacterial actin homologs, little is known about the archaeal counterparts. As a first step, we expressed a predicted actin homolog of the thermophilic archaeon Thermoplasma acidophilum, Ta0583, and determined its crystal structure at 2.1A resolution. Ta0583 is expressed as a soluble protein in T.acidophilum and is an active ATPase at physiological temperature. In vitro, Ta0583 forms sheets with spacings resembling the crystal lattice, indicating an inherent propensity to form filamentous structures. The fold of Ta0583 contains the core structure of actin and clearly belongs to the actin/Hsp70 superfamily of ATPases. Ta0583 is approximately equidistant from actin and MreB on the structural level, and combines features from both eubacterial actin homologs, MreB and ParM. The structure of Ta0583 co-crystallized with ADP indicates that the nucleotide binds at the interface between the subdomains of Ta0583 in a manner similar to that of actin. However, the conformation of the nucleotide observed in complex with Ta0583 clearly differs from that in complex with actin, but closely resembles the conformation of ParM-bound nucleotide. On the basis of sequence and structural homology, we suggest that Ta0583 derives from a ParM-like actin homolog that was once encoded by a plasmid and was transferred into a common ancestor of Thermoplasma and Ferroplasma. Intriguingly, both genera are characterized by the lack of a cell wall, and therefore Ta0583 could have a function in cellular organization.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fs/2fsj_consurf.spt"</scriptWhenChecked>
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2FSJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermoplasma_acidophilum Thermoplasma acidophilum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FSJ OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Crystal structure of an archaeal actin homolog., Roeben A, Kofler C, Nagy I, Nickell S, Hartl FU, Bracher A, J Mol Biol. 2006 Apr 21;358(1):145-56. Epub 2006 Feb 9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16500678 16500678]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fsj ConSurf].
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[[Category: Single protein]]
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<div style="clear:both"></div>
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[[Category: Thermoplasma acidophilum]]
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== References ==
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[[Category: Bracher, A.]]
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<references/>
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[[Category: Hartl, F Ulrich.]]
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__TOC__
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[[Category: Kofler, C.]]
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</StructureSection>
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[[Category: Nagy, I.]]
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[[Category: Large Structures]]
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[[Category: Nickell, S.]]
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[[Category: Thermoplasma acidophilum DSM 1728]]
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[[Category: Roeben, A.]]
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[[Category: Bracher A]]
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[[Category: Actin homolog]]
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[[Category: Kofler C]]
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[[Category: Archaea]]
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[[Category: Nagy I]]
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[[Category: Atpase]]
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[[Category: Nickell S]]
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[[Category: Mreb]]
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[[Category: Roeben A]]
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[[Category: Parm]]
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[[Category: Ulrich Hartl F]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:15:45 2008''
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Current revision

Crystal structure of Ta0583, an archaeal actin homolog, native data

PDB ID 2fsj

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