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| - | [[Image:2mm1.gif|left|200px]]<br /> | + | #REDIRECT [[3rgk]] This PDB entry is obsolete and replaced by 3rgk |
| - | <applet load="2mm1" size="450" color="white" frame="true" align="right" spinBox="true"
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| - | caption="2mm1, resolution 2.8Å" />
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| - | '''X-RAY CRYSTAL STRUCTURE OF A RECOMBINANT HUMAN MYOGLOBIN MUTANT AT 2.8 ANGSTROMS RESOLUTION'''<br />
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| - | ==Overview==
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| - | We have grown crystals in trigonal space group P3(2)21 of a mutant human, myoglobin, aquomet form, in which lysine at position 45 has been replaced, by arginine and cysteine at position 110 has been replaced by alanine., Suitable crystals of native recombinant human myoglobin have not been, obtained. We have used the molecular replacement method to determine the, X-ray crystal structure of the mutant at 2.8 A resolution. At the present, stage of refinement, the crystallographic R-value for the model, with, tightly restrained stereochemistry, is 0.158 for 5.0 to 2.8 A data. As, expected, the overall structure is quite similar to the sperm whale, myoglobin structure. Arginine 45 adopts a well-ordered conformation, similar to that found in aquomet sperm whale myoglobin.
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| - | ==Disease==
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| - | Known diseases associated with this structure: Chronic infections, due to MBL deficiency OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=154545 154545]], Diabetes mellitus, gestational, susceptibility to OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=154545 154545]], Mannose-binding protein deficiency OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=154545 154545]], Meningococcal disease, susceptibility to OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=154545 154545]]
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| - | ==About this Structure==
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| - | 2MM1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with HEM as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2MM1 OCA].
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| - | ==Reference==
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| - | X-ray crystal structure of a recombinant human myoglobin mutant at 2.8 A resolution., Hubbard SR, Hendrickson WA, Lambright DG, Boxer SG, J Mol Biol. 1990 May 20;213(2):215-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=2342104 2342104]
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| - | [[Category: Homo sapiens]]
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| - | [[Category: Single protein]]
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| - | [[Category: Boxer, S.G.]]
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| - | [[Category: Hendrickson, W.A.]]
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| - | [[Category: Hubbard, S.R.]]
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| - | [[Category: Lambright, D.G.]]
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| - | [[Category: HEM]]
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| - | [[Category: oxygen transport]]
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| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 22:58:43 2007''
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