2fu3

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[[Image:2fu3.gif|left|200px]]
 
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==Crystal structure of gephyrin E-domain==
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The line below this paragraph, containing "STRUCTURE_2fu3", creates the "Structure Box" on the page.
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<StructureSection load='2fu3' size='340' side='right'caption='[[2fu3]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2fu3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FU3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FU3 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fu3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fu3 OCA], [https://pdbe.org/2fu3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fu3 RCSB], [https://www.ebi.ac.uk/pdbsum/2fu3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fu3 ProSAT]</span></td></tr>
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{{STRUCTURE_2fu3| PDB=2fu3 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GEPH_RAT GEPH_RAT] Microtubule-associated protein involved in membrane protein-cytoskeleton interactions. It is thought to anchor the inhibitory glycine receptor (GLYR) to subsynaptic microtubules (By similarity). Catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released.<ref>PMID:8264797</ref> <ref>PMID:9990024</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fu/2fu3_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fu3 ConSurf].
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<div style="clear:both"></div>
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'''Crystal structure of gephyrin E-domain'''
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==See Also==
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*[[Gephyrin|Gephyrin]]
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== References ==
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==Overview==
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<references/>
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Glycine is the major inhibitory neurotransmitter in the spinal cord and brain stem. Gephyrin is required to achieve a high concentration of glycine receptors (GlyRs) in the postsynaptic membrane, which is crucial for efficient glycinergic signal transduction. The interaction between gephyrin and the GlyR involves the E-domain of gephyrin and a cytoplasmic loop located between transmembrane segments three and four of the GlyR beta subunit. Here, we present crystal structures of the gephyrin E-domain with and without the GlyR beta-loop at 2.4 and 2.7 A resolutions, respectively. The GlyR beta-loop is bound in a symmetric 'key and lock' fashion to each E-domain monomer in a pocket adjacent to the dimer interface. Structure-guided mutagenesis followed by in vitro binding and in vivo colocalization assays demonstrate that a hydrophobic interaction formed by Phe 330 of gephyrin and Phe 398 and Ile 400 of the GlyR beta-loop is crucial for binding.
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2FU3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FU3 OCA].
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==Reference==
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Deciphering the structural framework of glycine receptor anchoring by gephyrin., Kim EY, Schrader N, Smolinsky B, Bedet C, Vannier C, Schwarz G, Schindelin H, EMBO J. 2006 Mar 22;25(6):1385-95. Epub 2006 Mar 2. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16511563 16511563]
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[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
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[[Category: Single protein]]
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[[Category: Kim EY]]
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[[Category: Kim, E Y.]]
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[[Category: Schindelin H]]
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[[Category: Schindelin, H.]]
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[[Category: Gephyrin]]
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[[Category: Glycine receptor]]
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[[Category: Neuroreceptor anchoring]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:18:33 2008''
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Current revision

Crystal structure of gephyrin E-domain

PDB ID 2fu3

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