2nlw

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(New page: 200px<br /> <applet load="2nlw" size="450" color="white" frame="true" align="right" spinBox="true" caption="2nlw" /> '''Solution structure of the RRM domain of hum...)
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[[Image:2nlw.gif|left|200px]]<br />
 
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<applet load="2nlw" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2nlw" />
 
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'''Solution structure of the RRM domain of human eukaryotic initiation factor 3b'''<br />
 
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==Overview==
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==Solution structure of the RRM domain of human eukaryotic initiation factor 3b==
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Mammalian eIF3 is a 700 kDa multiprotein complex essential for initiation, of protein synthesis in eukaryotic cells. It consists of 13 subunits, (eIF3a-m), among which eIF3b serves as a major scaffolding protein. Here, we report the solution structure of the N-terminal RNA Recognition Motif, of human eIF3b (eIF3b-RRM) determined by NMR spectroscopy. The structure, reveals a non-canonical RRM with a negatively charged surface in the, beta-sheet area contradictory with potential RNA binding activity., Instead, eIF3j, which is required for stable 40S ribosome binding of the, eIF3 complex, specifically binds to the rear alpha-helices of the, eIF3b-RRM, opposite to its beta-sheet surface. Moreover, we identify that, an N-terminal 69 amino acid peptide of eIF3j is sufficient for binding to, eIF3b-RRM and that this interaction is essential for eIF3b-RRM recruitment, to the 40S ribosomal subunit. Our results provide the first structure of, an important subdomain of a core eIF3 subunit, and detailed insights into, protein-protein interactions between two eIF3 subunits required for stable, eIF3 recruitment to the 40S ribosome.
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<StructureSection load='2nlw' size='340' side='right'caption='[[2nlw]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2nlw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NLW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NLW FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2nlw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nlw OCA], [https://pdbe.org/2nlw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2nlw RCSB], [https://www.ebi.ac.uk/pdbsum/2nlw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2nlw ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/EIF3B_HUMAN EIF3B_HUMAN] Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.<ref>PMID:9388245</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nl/2nlw_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2nlw ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Mammalian eIF3 is a 700-kDa multiprotein complex essential for initiation of protein synthesis in eukaryotic cells. It consists of 13 subunits (eIF3a to -m), among which eIF3b serves as a major scaffolding protein. Here we report the solution structure of the N-terminal RNA recognition motif of human eIF3b (eIF3b-RRM) determined by NMR spectroscopy. The structure reveals a noncanonical RRM with a negatively charged surface in the beta-sheet area contradictory with potential RNA binding activity. Instead, eIF3j, which is required for stable 40 S ribosome binding of the eIF3 complex, specifically binds to the rear alpha-helices of the eIF3b-RRM, opposite to its beta-sheet surface. Moreover, we identify that an N-terminal 69-amino acid peptide of eIF3j is sufficient for binding to eIF3b-RRM and that this interaction is essential for eIF3b-RRM recruitment to the 40 S ribosomal subunit. Our results provide the first structure of an important subdomain of a core eIF3 subunit and detailed insights into protein-protein interactions between two eIF3 subunits required for stable eIF3 recruitment to the 40 S subunit.
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==About this Structure==
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Structure of eIF3b RNA recognition motif and its interaction with eIF3j: structural insights into the recruitment of eIF3b to the 40 S ribosomal subunit.,ElAntak L, Tzakos AG, Locker N, Lukavsky PJ J Biol Chem. 2007 Mar 16;282(11):8165-74. Epub 2006 Dec 26. PMID:17190833<ref>PMID:17190833</ref>
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2NLW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2NLW OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structure of eIF3b-RRM and its interaction with eIF3j: Structural insights into the recruitment of eIF3b to the 40S ribosomal subunit., Elantak L, Tzakos AG, Locker N, Lukavsky PJ, J Biol Chem. 2006 Dec 26;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17190833 17190833]
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</div>
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[[Category: Homo sapiens]]
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<div class="pdbe-citations 2nlw" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: ElAntak, L.]]
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[[Category: Locker, N.]]
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[[Category: Lukavsky, P.J.]]
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[[Category: Tzakos, A.G.]]
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[[Category: eukaryotic initiation factor 3 complex]]
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[[Category: rna recognition motif]]
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[[Category: translation initiation]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 22:59:23 2007''
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==See Also==
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*[[Eukaryotic initiation factor 3D structures|Eukaryotic initiation factor 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Homo sapiens]]
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[[Category: Large Structures]]
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[[Category: ElAntak L]]
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[[Category: Locker N]]
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[[Category: Lukavsky PJ]]
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[[Category: Tzakos AG]]

Current revision

Solution structure of the RRM domain of human eukaryotic initiation factor 3b

PDB ID 2nlw

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