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2nsu

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(New page: 200px<br /> <applet load="2nsu" size="450" color="white" frame="true" align="right" spinBox="true" caption="2nsu, resolution 27.0&Aring;" /> '''Crystal structure o...)
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[[Image:2nsu.gif|left|200px]]<br />
 
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<applet load="2nsu" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2nsu, resolution 27.0&Aring;" />
 
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'''Crystal structure of the ectodomain of human transferrin receptor fitted into a cryo-EM reconstruction of canine parvovirus and feline transferrin receptor complex'''<br />
 
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==Overview==
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==Crystal structure of the ectodomain of human transferrin receptor fitted into a cryo-EM reconstruction of canine parvovirus and feline transferrin receptor complex==
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Although many viruses are icosahedral when they initially bind to one or, more receptor molecules on the cell surface, such an interaction is, asymmetric, probably causing a breakdown in the symmetry and conformation, of the original infecting virion in preparation for membrane penetration, and release of the viral genome. Cryoelectron microscopy and biochemical, analyses show that transferrin receptor, the cellular receptor for canine, parvovirus, can bind to only one or a few of the 60 icosahedrally, equivalent sites on the virion, indicating that either canine parvovirus, has inherent asymmetry or binding of receptor induces asymmetry. The, asymmetry of receptor binding to canine parvovirus is reminiscent of the, special portal in tailed bacteriophages and some large, icosahedral, viruses. Asymmetric interactions of icosahedral viruses with their hosts, might be a more common phenomenon than previously thought and may have, been obscured by averaging in previous crystallographic and electron, microscopic structure determinations.
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<SX load='2nsu' size='340' side='right' viewer='molstar' caption='[[2nsu]], [[Resolution|resolution]] 27.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2nsu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NSU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NSU FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 27&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2nsu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nsu OCA], [https://pdbe.org/2nsu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2nsu RCSB], [https://www.ebi.ac.uk/pdbsum/2nsu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2nsu ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TFR1_HUMAN TFR1_HUMAN] Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site.<ref>PMID:3568132</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ns/2nsu_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2nsu ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Although many viruses are icosahedral when they initially bind to one or more receptor molecules on the cell surface, such an interaction is asymmetric, probably causing a breakdown in the symmetry and conformation of the original infecting virion in preparation for membrane penetration and release of the viral genome. Cryoelectron microscopy and biochemical analyses show that transferrin receptor, the cellular receptor for canine parvovirus, can bind to only one or a few of the 60 icosahedrally equivalent sites on the virion, indicating that either canine parvovirus has inherent asymmetry or binding of receptor induces asymmetry. The asymmetry of receptor binding to canine parvovirus is reminiscent of the special portal in tailed bacteriophages and some large, icosahedral viruses. Asymmetric interactions of icosahedral viruses with their hosts might be a more common phenomenon than previously thought and may have been obscured by averaging in previous crystallographic and electron microscopic structure determinations.
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==About this Structure==
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Asymmetric binding of transferrin receptor to parvovirus capsids.,Hafenstein S, Palermo LM, Kostyuchenko VA, Xiao C, Morais MC, Nelson CD, Bowman VD, Battisti AJ, Chipman PR, Parrish CR, Rossmann MG Proc Natl Acad Sci U S A. 2007 Apr 17;104(16):6585-9. Epub 2007 Apr 9. PMID:17420467<ref>PMID:17420467</ref>
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2NSU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2NSU OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Asymmetric binding of transferrin receptor to parvovirus capsids., Hafenstein S, Palermo LM, Kostyuchenko VA, Xiao C, Morais MC, Nelson CD, Bowman VD, Battisti AJ, Chipman PR, Parrish CR, Rossmann MG, Proc Natl Acad Sci U S A. 2007 Apr 17;104(16):6585-9. Epub 2007 Apr 9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17420467 17420467]
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</div>
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[[Category: Homo sapiens]]
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<div class="pdbe-citations 2nsu" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Hafenstein, S.]]
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[[Category: Kostyuchenko, V.A.]]
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[[Category: Rossmann, M.G.]]
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[[Category: transferrin receptor]]
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[[Category: virus-receptor complex]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 23:02:26 2007''
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==See Also==
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*[[Transferrin receptor|Transferrin receptor]]
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== References ==
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<references/>
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__TOC__
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</SX>
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[[Category: Homo sapiens]]
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[[Category: Large Structures]]
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[[Category: Hafenstein S]]
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[[Category: Kostyuchenko VA]]
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[[Category: Rossmann MG]]

Current revision

Crystal structure of the ectodomain of human transferrin receptor fitted into a cryo-EM reconstruction of canine parvovirus and feline transferrin receptor complex

2nsu, resolution 27.00Å

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