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2gho

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[[Image:2gho.gif|left|200px]]
 
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==Recombinant Thermus aquaticus RNA polymerase for Structural Studies==
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The line below this paragraph, containing "STRUCTURE_2gho", creates the "Structure Box" on the page.
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<StructureSection load='2gho' size='340' side='right'caption='[[2gho]], [[Resolution|resolution]] 5.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2gho]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GHO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GHO FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gho FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gho OCA], [https://pdbe.org/2gho PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gho RCSB], [https://www.ebi.ac.uk/pdbsum/2gho PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gho ProSAT]</span></td></tr>
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{{STRUCTURE_2gho| PDB=2gho | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RPOA_THEAQ RPOA_THEAQ]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gh/2gho_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gho ConSurf].
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<div style="clear:both"></div>
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'''Recombinant Thermus aquaticus RNA polymerase for Structural Studies'''
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==See Also==
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*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Advances in the structural biology of bacterial transcription have come from studies of RNA polymerases (RNAPs) from the thermophilic eubacteria Thermus aquaticus (Taq) and Thermus thermophilus (Tth). These structural studies have been limited by the fact that only endogenous Taq or Tth RNAP, laboriously purified from large quantities of Taq or Tth cell paste and offering few options for genetic modification, is suitable for structural studies. Recombinant systems for the preparation of Taq RNAP by co-overexpression and assembly in the heterologous host, Escherichia coli, have been described, but these did not yield enzyme suitable for crystallographic studies. Here we describe recombinant systems for the preparation of Taq RNAP harboring full or partial deletions of the Taq beta' non-conserved domain (NCD), yielding enzyme suitable for crystallographic studies. This opens the way for structural studies of genetically manipulated enzymes, allowing the preparation of more crystallizable enzymes and facilitating detailed structure/function analysis. Characterization of the Taqbeta'NCD deletion mutants generated in this study showed that the beta'NCD is important for the efficient binding of the sigma subunit, confirming previous hypotheses. Finally, preliminary structural analysis (at 4.1Angstroms resolution) of one of the recombinant mutants revealed a previously unobserved conformation of the beta-flap, further defining the range of conformations accessible to this flexible structural element.
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[[Category: Large Structures]]
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==About this Structure==
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2GHO is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GHO OCA].
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==Reference==
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Recombinant Thermus aquaticus RNA polymerase for structural studies., Kuznedelov K, Lamour V, Patikoglou G, Chlenov M, Darst SA, Severinov K, J Mol Biol. 2006 May 26;359(1):110-21. Epub 2006 Mar 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16618493 16618493]
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[[Category: DNA-directed RNA polymerase]]
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[[Category: Protein complex]]
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[[Category: Thermus aquaticus]]
[[Category: Thermus aquaticus]]
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[[Category: Darst, S A.]]
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[[Category: Darst SA]]
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[[Category: Lamour, V.]]
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[[Category: Lamour V]]
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[[Category: Coexpression]]
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[[Category: Recombinant multiprotein complex]]
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[[Category: Thermus aquaticus rna polymerase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:07:12 2008''
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Current revision

Recombinant Thermus aquaticus RNA polymerase for Structural Studies

PDB ID 2gho

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