2gif

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[[Image:2gif.gif|left|200px]]
 
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==Asymmetric structure of trimeric AcrB from Escherichia coli==
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The line below this paragraph, containing "STRUCTURE_2gif", creates the "Structure Box" on the page.
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<StructureSection load='2gif' size='340' side='right'caption='[[2gif]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2gif]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GIF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GIF FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr>
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{{STRUCTURE_2gif| PDB=2gif | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gif FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gif OCA], [https://pdbe.org/2gif PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gif RCSB], [https://www.ebi.ac.uk/pdbsum/2gif PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gif ProSAT]</span></td></tr>
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</table>
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'''Asymmetric structure of trimeric AcrB from Escherichia coli'''
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== Function ==
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[https://www.uniprot.org/uniprot/ACRB_ECOLI ACRB_ECOLI] AcrAB is a drug efflux protein with a broad substrate specificity.<ref>PMID:16915237</ref> <ref>PMID:16946072</ref> <ref>PMID:17194213</ref>
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gi/2gif_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gif ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
The AcrA/AcrB/TolC complex spans the inner and outer membranes of Escherichia coli and serves as its major drug-resistance pump. Driven by the proton motive force, it mediates the efflux of bile salts, detergents, organic solvents, and many structurally unrelated antibiotics. Here, we report a crystallographic structure of trimeric AcrB determined at 2.9 and 3.0 angstrom resolution in space groups that allow asymmetry of the monomers. This structure reveals three different monomer conformations representing consecutive states in a transport cycle. The structural data imply an alternating access mechanism and a novel peristaltic mode of drug transport by this type of transporter.
The AcrA/AcrB/TolC complex spans the inner and outer membranes of Escherichia coli and serves as its major drug-resistance pump. Driven by the proton motive force, it mediates the efflux of bile salts, detergents, organic solvents, and many structurally unrelated antibiotics. Here, we report a crystallographic structure of trimeric AcrB determined at 2.9 and 3.0 angstrom resolution in space groups that allow asymmetry of the monomers. This structure reveals three different monomer conformations representing consecutive states in a transport cycle. The structural data imply an alternating access mechanism and a novel peristaltic mode of drug transport by this type of transporter.
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==About this Structure==
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Structural asymmetry of AcrB trimer suggests a peristaltic pump mechanism.,Seeger MA, Schiefner A, Eicher T, Verrey F, Diederichs K, Pos KM Science. 2006 Sep 1;313(5791):1295-8. PMID:16946072<ref>PMID:16946072</ref>
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2GIF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GIF OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structural asymmetry of AcrB trimer suggests a peristaltic pump mechanism., Seeger MA, Schiefner A, Eicher T, Verrey F, Diederichs K, Pos KM, Science. 2006 Sep 1;313(5791):1295-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16946072 16946072]
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</div>
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<div class="pdbe-citations 2gif" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Diederichs, K.]]
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[[Category: Diederichs K]]
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[[Category: Eicher, T.]]
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[[Category: Eicher T]]
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[[Category: Pos, K M.]]
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[[Category: Pos KM]]
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[[Category: Schiefner, A.]]
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[[Category: Schiefner A]]
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[[Category: Seeger, M A.]]
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[[Category: Seeger MA]]
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[[Category: Verrey, F.]]
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[[Category: Verrey F]]
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[[Category: Alternating site mechanism]]
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[[Category: Antibiotic resistance]]
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[[Category: Drug-efflux pump]]
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[[Category: Membrane protein]]
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[[Category: Rnd]]
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[[Category: Secondary transport]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:08:31 2008''
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Asymmetric structure of trimeric AcrB from Escherichia coli

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