2gj2

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[[Image:2gj2.gif|left|200px]]
 
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==Crystal Structure of VP9 from White Spot Syndrome Virus==
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The line below this paragraph, containing "STRUCTURE_2gj2", creates the "Structure Box" on the page.
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<StructureSection load='2gj2' size='340' side='right'caption='[[2gj2]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2gj2]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Shrimp_white_spot_syndrome_virus Shrimp white spot syndrome virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GJ2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GJ2 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr>
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{{STRUCTURE_2gj2| PDB=2gj2 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gj2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gj2 OCA], [https://pdbe.org/2gj2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gj2 RCSB], [https://www.ebi.ac.uk/pdbsum/2gj2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gj2 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q91LD0_9VIRU Q91LD0_9VIRU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gj/2gj2_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gj2 ConSurf].
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<div style="clear:both"></div>
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'''Crystal Structure of VP9 from White Spot Syndrome Virus'''
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==See Also==
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*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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White spot syndrome virus (WSSV) is a major pathogen in shrimp aquaculture. VP9, a full-length protein of WSSV, encoded by open reading frame wsv230, was identified for the first time in the infected Penaeus monodon shrimp tissues, gill, and stomach as a novel, nonstructural protein by Western blotting, mass spectrometry, and immunoelectron microscopy. Real-time reverse transcription-PCR demonstrated that the transcription of VP9 started from the early to the late stage of WSSV infection as a major mRNA species. The structure of full-length VP9 was determined by both X-ray and nuclear magnetic resonance (NMR) techniques. It is the first structure to be reported for WSSV proteins. The crystal structure of VP9 revealed a ferredoxin fold with divalent metal ion binding sites. Cadmium sulfate was found to be essential for crystallization. The Cd2+ ions were bound between the monomer interfaces of the homodimer. Various divalent metal ions have been titrated against VP9, and their interactions were analyzed using NMR spectroscopy. The titration data indicated that VP9 binds with both Zn2+ and Cd2+. VP9 adopts a similar fold as the DNA binding domain of the papillomavirus E2 protein. Based on our present investigations, we hypothesize that VP9 might be involved in the transcriptional regulation of WSSV, a function similar to that of the E2 protein during papillomavirus infection of the host cells.
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[[Category: Large Structures]]
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==About this Structure==
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2GJ2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Shrimp_white_spot_syndrome_virus Shrimp white spot syndrome virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GJ2 OCA].
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==Reference==
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Identification of a novel nonstructural protein, VP9, from white spot syndrome virus: its structure reveals a ferredoxin fold with specific metal binding sites., Liu Y, Wu J, Song J, Sivaraman J, Hew CL, J Virol. 2006 Nov;80(21):10419-27. Epub 2006 Sep 6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16956937 16956937]
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[[Category: Shrimp white spot syndrome virus]]
[[Category: Shrimp white spot syndrome virus]]
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[[Category: Single protein]]
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[[Category: Hew CL]]
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[[Category: Hew, C L.]]
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[[Category: Liu Y]]
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[[Category: Liu, Y.]]
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[[Category: Sivaraman J]]
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[[Category: Sivaraman, J.]]
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[[Category: Song JX]]
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[[Category: Song, J X.]]
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[[Category: Wu JL]]
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[[Category: Wu, J L.]]
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[[Category: Ferredoxin fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:09:47 2008''
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Current revision

Crystal Structure of VP9 from White Spot Syndrome Virus

PDB ID 2gj2

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