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| - | [[Image:2gmg.gif|left|200px]] | |
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| - | <!--
| + | ==Solution NMR Structure of protein PF0610 from Pyrococcus furiosus, Northeast Structural Genomics Consortium Target PfG3== |
| - | The line below this paragraph, containing "STRUCTURE_2gmg", creates the "Structure Box" on the page.
| + | <StructureSection load='2gmg' size='340' side='right'caption='[[2gmg]]' scene=''> |
| - | You may change the PDB parameter (which sets the PDB file loaded into the applet)
| + | == Structural highlights == |
| - | or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| + | <table><tr><td colspan='2'>[[2gmg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus_DSM_3638 Pyrococcus furiosus DSM 3638]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GMG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GMG FirstGlance]. <br> |
| - | or leave the SCENE parameter empty for the default display.
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | -->
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gmg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gmg OCA], [https://pdbe.org/2gmg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gmg RCSB], [https://www.ebi.ac.uk/pdbsum/2gmg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gmg ProSAT], [https://www.topsan.org/Proteins/NESGC/2gmg TOPSAN]</span></td></tr> |
| - | {{STRUCTURE_2gmg| PDB=2gmg | SCENE= }}
| + | </table> |
| - | | + | == Function == |
| - | '''Solution NMR Structure of protein PF0610 from Pyrococcus furiosus, Northeast Structural Genomics Consortium Target PfG3'''
| + | [https://www.uniprot.org/uniprot/Q8U362_PYRFU Q8U362_PYRFU] |
| - | | + | == Evolutionary Conservation == |
| - | | + | [[Image:Consurf_key_small.gif|200px|right]] |
| - | ==Overview== | + | Check<jmol> |
| - | PF0610, a protein from the hyperthermophile Pyrococcus furiosus, has homologues only in other archaeal species and in three species of Fe(III)-reducing bacteria. It is thought to have a helix-turn-helix (HTH) domain at the N-terminus and possesses two CXXC motifs characteristic of metal binding proteins. We have determined the solution structure of the Zn-bound protein using NMR. PF0610 is a novel winged helix-turn-helix (wHTH) protein with a rubredoxin-like Zn ribbon as its W1 segment. In addition, it possesses a large number of basic residues on its surface. Clusters of basic residues can be found on both helix H3 and the metal-binding loops of W1, suggesting that it might be a DNA-binding protein. Accordingly, gel shift assays using both linear and circular DNA showed that PF0610 does bind DNA, at least in a sequence-independent fashion. Modeling the PF0610-DNA interaction based on other wHTH protein-DNA structures revealed that besides helix H3, basic residues around the second CXXC motif in the metal-binding loop could make extensive contacts with DNA. However, the bulkiness of the W1 region implies that the DNA conformation may be distorted upon PF0610 binding. PF0610 is the first protein known to have a Zn ribbon-embedded wHTH fold and, as such, has potential roles both as a metal-dependent transcription regulator and as a component of the chromosome packing system in P. furiosus. The discovery of this novel structure represents the addition of another branch to the winged HTH protein family and could contribute to our understanding of transcription regulatory processes in P. furiosus.
| + | <jmolCheckbox> |
| - | | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gm/2gmg_consurf.spt"</scriptWhenChecked> |
| - | ==About this Structure== | + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| - | 2GMG is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus_dsm_3638 Pyrococcus furiosus dsm 3638]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GMG OCA].
| + | <text>to colour the structure by Evolutionary Conservation</text> |
| - | | + | </jmolCheckbox> |
| - | ==Reference== | + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gmg ConSurf]. |
| - | PF0610, a novel winged helix-turn-helix variant possessing a rubredoxin-like Zn ribbon motif from the hyperthermophilic archaeon, Pyrococcus furiosus., Wang X, Lee HS, Sugar FJ, Jenney FE Jr, Adams MW, Prestegard JH, Biochemistry. 2007 Jan 23;46(3):752-61. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17223696 17223696]
| + | <div style="clear:both"></div> |
| - | [[Category: Pyrococcus furiosus dsm 3638]] | + | __TOC__ |
| - | [[Category: Single protein]] | + | </StructureSection> |
| - | [[Category: Adams, M W.]] | + | [[Category: Large Structures]] |
| - | [[Category: Lee, H S.]] | + | [[Category: Pyrococcus furiosus DSM 3638]] |
| - | [[Category: Montelione, G T.]]
| + | [[Category: Adams MW]] |
| - | [[Category: NESG, Northeast Structural Genomics Consortium.]] | + | [[Category: Lee HS]] |
| - | [[Category: Prestegard, J H.]] | + | [[Category: Montelione GT]] |
| - | [[Category: Wang, X.]] | + | [[Category: Prestegard JH]] |
| - | [[Category: Dna binding protein]] | + | [[Category: Wang X]] |
| - | [[Category: Nesg]] | + | |
| - | [[Category: Northeast structural genomics consortium]] | + | |
| - | [[Category: Protein structure initiative]] | + | |
| - | [[Category: Psi]] | + | |
| - | [[Category: Structural genomic]] | + | |
| - | [[Category: Winged-helix like protein with metal binding site]] | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:16:13 2008''
| + | |
