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| - | [[Image:2goz.gif|left|200px]] | + | #REDIRECT [[3zd5]] This PDB entry is obsolete and replaced by 3zd5 |
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| - | {{STRUCTURE_2goz| PDB=2goz | SCENE= }}
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| - | '''The 2.2 A structure of a full-length catalytically active hammerhead ribozyme'''
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| - | ==Overview==
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| - | Minimal hammerhead ribozymes have been characterized extensively by static and time-resolved crystallography as well as numerous biochemical analyses, leading to mutually contradictory mechanistic explanations for catalysis. We present the 2.2 A resolution crystal structure of a full-length Schistosoma mansoni hammerhead ribozyme that permits us to explain the structural basis for its 1000-fold catalytic enhancement. The full-length hammerhead structure reveals how tertiary interactions occurring remotely from the active site prime this ribozyme for catalysis. G-12 and G-8 are positioned consistent with their previously suggested roles in acid-base catalysis, the nucleophile is aligned with a scissile phosphate positioned proximal to the A-9 phosphate, and previously unexplained roles of other conserved nucleotides become apparent within the context of a distinctly new fold that nonetheless accommodates the previous structural studies. These interactions permit us to explain the previously irreconcilable sets of experimental results in a unified, consistent, and unambiguous manner.
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| - | ==About this Structure==
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| - | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GOZ OCA].
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| - | ==Reference==
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| - | Tertiary contacts distant from the active site prime a ribozyme for catalysis., Martick M, Scott WG, Cell. 2006 Jul 28;126(2):309-20. Epub 2006 Jul 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16859740 16859740]
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| - | [[Category: Martick, M.]]
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| - | [[Category: Scott, W G.]]
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| - | [[Category: A-form helix]]
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| - | [[Category: Catalytic rna]]
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| - | [[Category: Hammerhead ribozyme]]
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| - | [[Category: Hammerhead rna]]
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| - | [[Category: In-line attack]]
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| - | [[Category: Ribozyme]]
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| - | [[Category: Stem-loop interaction]]
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| - | [[Category: Uridine turn]]
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:21:14 2008''
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