2grw

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[[Image:2grw.gif|left|200px]]
 
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==Solution structure of the poliovirus 3'-UTR Y-stem==
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The line below this paragraph, containing "STRUCTURE_2grw", creates the "Structure Box" on the page.
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<StructureSection load='2grw' size='340' side='right'caption='[[2grw]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2grw]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GRW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GRW FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2grw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2grw OCA], [https://pdbe.org/2grw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2grw RCSB], [https://www.ebi.ac.uk/pdbsum/2grw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2grw ProSAT]</span></td></tr>
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{{STRUCTURE_2grw| PDB=2grw | SCENE= }}
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</table>
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<div style="background-color:#fffaf0;">
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'''Solution structure of the poliovirus 3'-UTR Y-stem'''
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== Publication Abstract from PubMed ==
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==Overview==
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The previously described NMR structure of a 5'-CU-3'/5'-UU-3' motif, which is highly conserved within the 3'-UTR Y-stem of poliovirus-like enteroviruses, revealed striking regularities of the local helix geometry, thus retaining the pseudo-twofold symmetry of the RNA helix. A mutant virus with both pyrimidine base pairs changed into Watson-Crick replicated as wild type, indicating the functional importance of this symmetry relation in viral RNA replication. Here we investigated the effect of changing only one of the two pyrimidine base pairs to Watson-Crick. We determined the NMR structures of two Y-stem variants: one containing the 5'-CU-3'/5'-AU-3' motif, which has been found in wild-type virus isolates as well, and the other containing a 5'-CU-3'/5'-UG-3' motif, which is not present in any enterovirus sequenced to date. Both structures show single pyrimidine mismatches with intercalated bases. In the 5'-CU-3'/5'-AU-3' motif a C-U Watson-Crick-type base pair is formed that retains the pseudo-twofold symmetry, while in the 5'-CU-3'/5'-UG-3' motif a single asymmetric U-U mismatch breaks the twofold symmetry. Surprisingly, for the nonnatural variant no effect of the single base-pair replacement was observed on polioviral RNA replication using an in vitro replicon assay.
The previously described NMR structure of a 5'-CU-3'/5'-UU-3' motif, which is highly conserved within the 3'-UTR Y-stem of poliovirus-like enteroviruses, revealed striking regularities of the local helix geometry, thus retaining the pseudo-twofold symmetry of the RNA helix. A mutant virus with both pyrimidine base pairs changed into Watson-Crick replicated as wild type, indicating the functional importance of this symmetry relation in viral RNA replication. Here we investigated the effect of changing only one of the two pyrimidine base pairs to Watson-Crick. We determined the NMR structures of two Y-stem variants: one containing the 5'-CU-3'/5'-AU-3' motif, which has been found in wild-type virus isolates as well, and the other containing a 5'-CU-3'/5'-UG-3' motif, which is not present in any enterovirus sequenced to date. Both structures show single pyrimidine mismatches with intercalated bases. In the 5'-CU-3'/5'-AU-3' motif a C-U Watson-Crick-type base pair is formed that retains the pseudo-twofold symmetry, while in the 5'-CU-3'/5'-UG-3' motif a single asymmetric U-U mismatch breaks the twofold symmetry. Surprisingly, for the nonnatural variant no effect of the single base-pair replacement was observed on polioviral RNA replication using an in vitro replicon assay.
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==About this Structure==
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Breaking pseudo-twofold symmetry in the poliovirus 3'-UTR Y-stem by restoring Watson-Crick base pairs.,Zoll J, Tessari M, Van Kuppeveld FJ, Melchers WJ, Heus HA RNA. 2007 May;13(5):781-92. PMID:17449731<ref>PMID:17449731</ref>
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GRW OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Breaking pseudo-twofold symmetry in the poliovirus 3'-UTR Y-stem by restoring Watson-Crick base pairs., Zoll J, Tessari M, Van Kuppeveld FJ, Melchers WJ, Heus HA, RNA. 2007 May;13(5):781-92. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17449731 17449731]
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</div>
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[[Category: Heus, H A.]]
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<div class="pdbe-citations 2grw" style="background-color:#fffaf0;"></div>
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[[Category: Kuppeveld, F J.M van.]]
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== References ==
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[[Category: Melchers, W J.G.]]
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<references/>
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[[Category: Tessari, M.]]
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__TOC__
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[[Category: Zoll, J.]]
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</StructureSection>
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[[Category: Poliovirus]]
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[[Category: Large Structures]]
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[[Category: Pseudo-2-fold symmetry]]
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[[Category: Heus HA]]
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[[Category: Rna]]
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[[Category: Melchers WJG]]
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[[Category: Stem-loop]]
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[[Category: Tessari M]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:26:54 2008''
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[[Category: Zoll J]]
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[[Category: Van Kuppeveld FJM]]

Current revision

Solution structure of the poliovirus 3'-UTR Y-stem

PDB ID 2grw

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