2gsq

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[[Image:2gsq.jpg|left|200px]]
 
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==GLUTATHIONE S-TRANSFERASE FROM SQUID DIGESTIVE GLAND COMPLEXED WITH S-(3-IODOBENZYL)GLUTATHIONE==
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The line below this paragraph, containing "STRUCTURE_2gsq", creates the "Structure Box" on the page.
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<StructureSection load='2gsq' size='340' side='right'caption='[[2gsq]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2gsq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Nototodarus_sloanii Nototodarus sloanii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GSQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GSQ FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GBI:S-(3-IODOBENZYL)GLUTATHIONE'>GBI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_2gsq| PDB=2gsq | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gsq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gsq OCA], [https://pdbe.org/2gsq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gsq RCSB], [https://www.ebi.ac.uk/pdbsum/2gsq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gsq ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GST_NOTSL GST_NOTSL] High activity toward 1-chloro-2,4-dinitrobenzene. Not very efficient at catalyzing the addition of GSH to enones and epoxides.<ref>PMID:7727393</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gs/2gsq_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gsq ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of the sigma class glutathione transferase from squid digestive gland in complex with S-(3-iodobenzyl)glutathione reveals a third binding site for the glutathione conjugate besides the two in the active sites of the dimer. The additional binding site is near the crystallographic two-fold axis between the two alpha 4-turn-alpha 5 motifs. The principal binding interactions with the conjugate include specific electrostatic interactions between the peptide and the two subunits and a hydrophobic cavity found across the two-fold axis that accommodates the 3-iodobenzyl group. Thus, two identical, symmetry-related but mutually exclusive binding modes for the third conjugate are observed. The hydrophobic pocket is about 14 A from the hydroxyl group of Tyr-7 in the active site. This site is a potential transport binding site for hydrophobic molecules or their glutathione conjugates.
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'''GLUTATHIONE S-TRANSFERASE FROM SQUID DIGESTIVE GLAND COMPLEXED WITH S-(3-IODOBENZYL)GLUTATHIONE'''
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Location of a potential transport binding site in a sigma class glutathione transferase by x-ray crystallography.,Ji X, von Rosenvinge EC, Johnson WW, Armstrong RN, Gilliland GL Proc Natl Acad Sci U S A. 1996 Aug 6;93(16):8208-13. PMID:8710848<ref>PMID:8710848</ref>
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==Overview==
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The crystal structure of the sigma class glutathione transferase from squid digestive gland in complex with S-(3-iodobenzyl)glutathione reveals a third binding site for the glutathione conjugate besides the two in the active sites of the dimer. The additional binding site is near the crystallographic two-fold axis between the two alpha 4-turn-alpha 5 motifs. The principal binding interactions with the conjugate include specific electrostatic interactions between the peptide and the two subunits and a hydrophobic cavity found across the two-fold axis that accommodates the 3-iodobenzyl group. Thus, two identical, symmetry-related but mutually exclusive binding modes for the third conjugate are observed. The hydrophobic pocket is about 14 A from the hydroxyl group of Tyr-7 in the active site. This site is a potential transport binding site for hydrophobic molecules or their glutathione conjugates.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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2GSQ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Ommastrephes_sloani Ommastrephes sloani]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GSQ OCA].
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</div>
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<div class="pdbe-citations 2gsq" style="background-color:#fffaf0;"></div>
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==Reference==
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==See Also==
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Location of a potential transport binding site in a sigma class glutathione transferase by x-ray crystallography., Ji X, von Rosenvinge EC, Johnson WW, Armstrong RN, Gilliland GL, Proc Natl Acad Sci U S A. 1996 Aug 6;93(16):8208-13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8710848 8710848]
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*[[Glutathione S-transferase 3D structures|Glutathione S-transferase 3D structures]]
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[[Category: Glutathione transferase]]
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== References ==
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[[Category: Ommastrephes sloani]]
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<references/>
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[[Category: Single protein]]
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__TOC__
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[[Category: Armstrong, R N.]]
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</StructureSection>
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[[Category: Gilliland, G L.]]
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[[Category: Large Structures]]
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[[Category: Ji, X.]]
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[[Category: Nototodarus sloanii]]
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[[Category: Sigma class]]
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[[Category: Armstrong RN]]
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[[Category: Squid digestive gland]]
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[[Category: Gilliland GL]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:29:08 2008''
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[[Category: Ji X]]

Current revision

GLUTATHIONE S-TRANSFERASE FROM SQUID DIGESTIVE GLAND COMPLEXED WITH S-(3-IODOBENZYL)GLUTATHIONE

PDB ID 2gsq

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