This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2gt1

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:29, 14 February 2024) (edit) (undo)
 
(11 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2gt1.jpg|left|200px]]
 
-
<!--
+
==E. coli heptosyltransferase WaaC.==
-
The line below this paragraph, containing "STRUCTURE_2gt1", creates the "Structure Box" on the page.
+
<StructureSection load='2gt1' size='340' side='right'caption='[[2gt1]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2gt1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_UTI89 Escherichia coli UTI89]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GT1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GT1 FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
-
-->
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gt1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gt1 OCA], [https://pdbe.org/2gt1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gt1 RCSB], [https://www.ebi.ac.uk/pdbsum/2gt1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gt1 ProSAT]</span></td></tr>
-
{{STRUCTURE_2gt1| PDB=2gt1 | SCENE= }}
+
</table>
-
 
+
== Function ==
-
'''E. coli heptosyltransferase WaaC.'''
+
[https://www.uniprot.org/uniprot/Q1R4X0_ECOUT Q1R4X0_ECOUT]
-
 
+
== Evolutionary Conservation ==
-
 
+
[[Image:Consurf_key_small.gif|200px|right]]
-
==Overview==
+
Check<jmol>
-
Lipopolysaccharides constitute the outer leaflet of the outer membrane of Gram-negative bacteria and are therefore essential for cell growth and viability. The heptosyltransferase WaaC is a glycosyltransferase (GT) involved in the synthesis of the inner core region of LPS. It catalyzes the addition of the first L-glycero-D-manno-heptose (heptose) molecule to one 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) residue of the Kdo2-lipid A molecule. Heptose is an essential component of the LPS core domain; its absence results in a truncated lipopolysaccharide associated with the deep-rough phenotype causing a greater susceptibility to antibiotic and an attenuated virulence for pathogenic Gram-negative bacteria. Thus, WaaC represents a promising target in antibacterial drug design. Here, we report the structure of WaaC from the Escherichia coli pathogenic strain RS218 alone at 1.9 A resolution, and in complex with either ADP or the non-cleavable analog ADP-2-deoxy-2-fluoro-heptose of the sugar donor at 2.4 A resolution. WaaC adopts the GT-B fold in two domains, characteristic of one glycosyltransferase structural superfamily. The comparison of the three different structures shows that WaaC does not undergo a domain rotation, characteristic of the GT-B family, upon substrate binding, but allows the substrate analog and the reaction product to adopt remarkably distinct conformations inside the active site. In addition, both binary complexes offer a close view of the donor subsite and, together with results from site-directed mutagenesis studies, provide evidence for a model of the catalytic mechanism.
+
<jmolCheckbox>
-
 
+
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gt/2gt1_consurf.spt"</scriptWhenChecked>
-
==About this Structure==
+
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-
2GT1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_uti89 Escherichia coli uti89]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GT1 OCA].
+
<text>to colour the structure by Evolutionary Conservation</text>
-
 
+
</jmolCheckbox>
-
==Reference==
+
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gt1 ConSurf].
-
Structure of the Escherichia coli heptosyltransferase WaaC: binary complexes with ADP and ADP-2-deoxy-2-fluoro heptose., Grizot S, Salem M, Vongsouthi V, Durand L, Moreau F, Dohi H, Vincent S, Escaich S, Ducruix A, J Mol Biol. 2006 Oct 20;363(2):383-94. Epub 2006 Jul 29. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16963083 16963083]
+
<div style="clear:both"></div>
-
[[Category: Escherichia coli uti89]]
+
__TOC__
-
[[Category: Single protein]]
+
</StructureSection>
-
[[Category: Dohi, H.]]
+
[[Category: Escherichia coli UTI89]]
-
[[Category: Ducruix, A.]]
+
[[Category: Large Structures]]
-
[[Category: Durand, L.]]
+
[[Category: Dohi H]]
-
[[Category: Escaich, S.]]
+
[[Category: Ducruix A]]
-
[[Category: Grizot, S.]]
+
[[Category: Durand L]]
-
[[Category: Moreau, F.]]
+
[[Category: Escaich S]]
-
[[Category: Salem, M.]]
+
[[Category: Grizot S]]
-
[[Category: Vincent, S.]]
+
[[Category: Moreau F]]
-
[[Category: Vongsouthi, V.]]
+
[[Category: Salem M]]
-
[[Category: Gt-b fold]]
+
[[Category: Vincent S]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:29:49 2008''
+
[[Category: Vongsouthi V]]

Current revision

E. coli heptosyltransferase WaaC.

PDB ID 2gt1

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2gt1"
Personal tools