2gwc

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[[Image:2gwc.gif|left|200px]]
 
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==Crystal structure of plant glutamate cysteine ligase in complex with a transition state analogue==
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The line below this paragraph, containing "STRUCTURE_2gwc", creates the "Structure Box" on the page.
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<StructureSection load='2gwc' size='340' side='right'caption='[[2gwc]], [[Resolution|resolution]] 2.18&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2gwc]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Brassica_juncea Brassica juncea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GWC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GWC FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.18&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BSC:(S)-2-AMINO-4-(AMINO-BUTYLHYDROSULFINYL)BUTANOIC+ACID'>BSC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_2gwc| PDB=2gwc | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gwc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gwc OCA], [https://pdbe.org/2gwc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gwc RCSB], [https://www.ebi.ac.uk/pdbsum/2gwc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gwc ProSAT]</span></td></tr>
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</table>
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'''Crystal structure of plant glutamate cysteine ligase in complex with a transition state analogue'''
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== Function ==
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[https://www.uniprot.org/uniprot/GSH1_BRAJU GSH1_BRAJU] Participates in the detoxification process.<ref>PMID:9620267</ref> <ref>PMID:9119067</ref>
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gw/2gwc_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gwc ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
Glutathione (GSH) plays a crucial role in plant metabolism and stress response. The rate-limiting step in the biosynthesis of GSH is catalyzed by glutamate cysteine ligase (GCL) the activity of which is tightly regulated. The regulation of plant GCLs is poorly understood. The crystal structure of substrate-bound GCL from Brassica juncea at 2.1-A resolution reveals a plant-unique regulatory mechanism based on two intramolecular redox-sensitive disulfide bonds. Reduction of one disulfide bond allows a beta-hairpin motif to shield the active site of B. juncea GCL, thereby preventing the access of substrates. Reduction of the second disulfide bond reversibly controls dimer to monomer transition of B. juncea GCL that is associated with a significant inactivation of the enzyme. These regulatory events provide a molecular link between high GSH levels in the plant cell and associated down-regulation of its biosynthesis. Furthermore, known mutations in the Arabidopsis GCL gene affect residues in the close proximity of the active site and thus explain the decreased GSH levels in mutant plants. In particular, the mutation in rax1-1 plants causes impaired binding of cysteine.
Glutathione (GSH) plays a crucial role in plant metabolism and stress response. The rate-limiting step in the biosynthesis of GSH is catalyzed by glutamate cysteine ligase (GCL) the activity of which is tightly regulated. The regulation of plant GCLs is poorly understood. The crystal structure of substrate-bound GCL from Brassica juncea at 2.1-A resolution reveals a plant-unique regulatory mechanism based on two intramolecular redox-sensitive disulfide bonds. Reduction of one disulfide bond allows a beta-hairpin motif to shield the active site of B. juncea GCL, thereby preventing the access of substrates. Reduction of the second disulfide bond reversibly controls dimer to monomer transition of B. juncea GCL that is associated with a significant inactivation of the enzyme. These regulatory events provide a molecular link between high GSH levels in the plant cell and associated down-regulation of its biosynthesis. Furthermore, known mutations in the Arabidopsis GCL gene affect residues in the close proximity of the active site and thus explain the decreased GSH levels in mutant plants. In particular, the mutation in rax1-1 plants causes impaired binding of cysteine.
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==About this Structure==
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Structural basis for the redox control of plant glutamate cysteine ligase.,Hothorn M, Wachter A, Gromes R, Stuwe T, Rausch T, Scheffzek K J Biol Chem. 2006 Sep 15;281(37):27557-65. Epub 2006 Jun 9. PMID:16766527<ref>PMID:16766527</ref>
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2GWC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Brassica_juncea Brassica juncea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GWC OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structural basis for the redox control of plant glutamate cysteine ligase., Hothorn M, Wachter A, Gromes R, Stuwe T, Rausch T, Scheffzek K, J Biol Chem. 2006 Sep 15;281(37):27557-65. Epub 2006 Jun 9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16766527 16766527]
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</div>
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<div class="pdbe-citations 2gwc" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Brassica juncea]]
[[Category: Brassica juncea]]
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[[Category: Glutamate--cysteine ligase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Gromes R]]
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[[Category: Gromes, R.]]
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[[Category: Hothorn M]]
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[[Category: Hothorn, M.]]
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[[Category: Rausch T]]
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[[Category: Rausch, T.]]
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[[Category: Scheffzek K]]
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[[Category: Scheffzek, K.]]
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[[Category: Stuwe T]]
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[[Category: Stuwe, T.]]
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[[Category: Wachter A]]
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[[Category: Wachter, A.]]
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[[Category: Beta-hairpin]]
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[[Category: Disulfide bridge]]
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[[Category: Glutathione biosynthesis]]
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[[Category: Redox regulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:36:56 2008''
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Current revision

Crystal structure of plant glutamate cysteine ligase in complex with a transition state analogue

PDB ID 2gwc

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