2gxu

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[[Image:2gxu.gif|left|200px]]
 
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==HERA N-terminal domain in complex with orthophosphate, crystal form 1==
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The line below this paragraph, containing "STRUCTURE_2gxu", creates the "Structure Box" on the page.
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<StructureSection load='2gxu' size='340' side='right'caption='[[2gxu]], [[Resolution|resolution]] 1.67&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2gxu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB27 Thermus thermophilus HB27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GXU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GXU FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.67&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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{{STRUCTURE_2gxu| PDB=2gxu | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gxu OCA], [https://pdbe.org/2gxu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gxu RCSB], [https://www.ebi.ac.uk/pdbsum/2gxu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gxu ProSAT]</span></td></tr>
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</table>
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'''HERA N-terminal domain in complex with orthophosphate, crystal form 1'''
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== Function ==
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[https://www.uniprot.org/uniprot/O07897_THETH O07897_THETH]
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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DEAD box RNA helicases use the energy of ATP hydrolysis to unwind double-stranded RNA regions or to disrupt RNA/protein complexes. A minimal RNA helicase comprises nine conserved motifs distributed over two RecA-like domains. The N-terminal domain contains all motifs involved in nucleotide binding, namely the Q-motif, the DEAD box, and the P-loop, as well as the SAT motif, which has been implicated in the coordination of ATP hydrolysis and RNA unwinding. We present here the crystal structure of the N-terminal domain of the Thermus thermophilus RNA helicase Hera in complex with adenosine monophosphate (AMP). Upon binding of AMP the P-loop adopts a partially collapsed or half-open conformation that is still connected to the DEAD box motif, and the DEAD box in turn is linked to the SAT motif via hydrogen bonds. This network of interactions communicates changes in the P-loop conformation to distant parts of the helicase. The affinity of AMP is comparable to that of ADP and ATP, substantiating that the binding energy from additional phosphate moieties is directly converted into conformational changes of the entire helicase. Importantly, the N-terminal Hera domain forms a dimer in the crystal similar to that seen in another thermophilic prokaryote. It is possible that this mode of dimerization represents the prototypic architecture in RNA helicases of thermophilic origin.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gx/2gxu_consurf.spt"</scriptWhenChecked>
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2GXU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GXU OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Crystal structure and nucleotide binding of the Thermus thermophilus RNA helicase Hera N-terminal domain., Rudolph MG, Heissmann R, Wittmann JG, Klostermeier D, J Mol Biol. 2006 Aug 25;361(4):731-43. Epub 2006 Jul 12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16890241 16890241]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gxu ConSurf].
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[[Category: Single protein]]
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<div style="clear:both"></div>
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[[Category: Thermus thermophilus]]
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__TOC__
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[[Category: Klostermeier, D.]]
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</StructureSection>
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[[Category: Rudolph, M G.]]
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[[Category: Large Structures]]
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[[Category: Amp complex]]
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[[Category: Thermus thermophilus HB27]]
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[[Category: Atomic resolution]]
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[[Category: Klostermeier D]]
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[[Category: Ribosome biogenesis]]
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[[Category: Rudolph MG]]
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[[Category: Rna helicase]]
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[[Category: Thermophilic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:39:00 2008''
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Current revision

HERA N-terminal domain in complex with orthophosphate, crystal form 1

PDB ID 2gxu

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