This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2oot

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /> <applet load="2oot" size="450" color="white" frame="true" align="right" spinBox="true" caption="2oot, resolution 1.64&Aring;" /> '''A High Resolution S...)
Current revision (10:46, 30 August 2023) (edit) (undo)
 
(15 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2oot.gif|left|200px]]<br />
 
-
<applet load="2oot" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="2oot, resolution 1.64&Aring;" />
 
-
'''A High Resolution Structure of Ligand-free Human Glutamate Carboxypeptidase II'''<br />
 
-
==Overview==
+
==A High Resolution Structure of Ligand-free Human Glutamate Carboxypeptidase II==
-
Human glutamate carboxypeptidase II (GCPII; EC 3.4.17.21) is an, established marker for prostate-cancer diagnosis as well as a candidate, therapeutic target for the treatment of diverse pathologies that involve, glutamatergic transmission. Structural data on GCPII are thus valuable for, the design and optimization of GCPII-specific inhibitors and diagnostic, probes. The currently available structure of ligand-free GCPII was refined, to a resolution of 3.5 A. This work reports the structure of the protein, refined to 1.65 A resolution, with crystallographic values of R = 0.207, and R(free) = 0.228. The new structure extends the resolution appreciably, and the new model based on this data shows significant differences when, compared with the previously published model.
+
<StructureSection load='2oot' size='340' side='right'caption='[[2oot]], [[Resolution|resolution]] 1.64&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2oot]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OOT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OOT FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.64&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2oot FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2oot OCA], [https://pdbe.org/2oot PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2oot RCSB], [https://www.ebi.ac.uk/pdbsum/2oot PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2oot ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/FOLH1_HUMAN FOLH1_HUMAN] Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Isoform PSM-4 and isoform PSM-5 would appear to be physiologically irrelevant. Involved in prostate tumor progression. Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oo/2oot_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2oot ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Human glutamate carboxypeptidase II (GCPII; EC 3.4.17.21) is an established marker for prostate-cancer diagnosis as well as a candidate therapeutic target for the treatment of diverse pathologies that involve glutamatergic transmission. Structural data on GCPII are thus valuable for the design and optimization of GCPII-specific inhibitors and diagnostic probes. The currently available structure of ligand-free GCPII was refined to a resolution of 3.5 A. This work reports the structure of the protein refined to 1.65 A resolution, with crystallographic values of R = 0.207 and R(free) = 0.228. The new structure extends the resolution appreciably and the new model based on this data shows significant differences when compared with the previously published model.
-
==Disease==
+
A high-resolution structure of ligand-free human glutamate carboxypeptidase II.,Barinka C, Starkova J, Konvalinka J, Lubkowski J Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Mar 1;63(Pt, 3):150-3. Epub 2007 Feb 13. PMID:17329803<ref>PMID:17329803</ref>
-
Known diseases associated with this structure: Myocardial infarcation, susceptibility to OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=602855 602855]]
+
-
==About this Structure==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
2OOT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with NAG, ZN, CA and CL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Glutamate_carboxypeptidase_II Glutamate carboxypeptidase II], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.17.21 3.4.17.21] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2OOT OCA].
+
</div>
 +
<div class="pdbe-citations 2oot" style="background-color:#fffaf0;"></div>
-
==Reference==
+
==See Also==
-
A high-resolution structure of ligand-free human glutamate carboxypeptidase II., Barinka C, Starkova J, Konvalinka J, Lubkowski J, Acta Crystallograph Sect F Struct Biol Cryst Commun. 2007 Mar 1;63(Pt, 3):150-3. Epub 2007 Feb 13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17329803 17329803]
+
*[[Carboxypeptidase 3D structures|Carboxypeptidase 3D structures]]
-
[[Category: Glutamate carboxypeptidase II]]
+
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Barinka, C.]]
+
[[Category: Barinka C]]
-
[[Category: Lubkowski, J.]]
+
[[Category: Lubkowski J]]
-
[[Category: CA]]
+
-
[[Category: CL]]
+
-
[[Category: NAG]]
+
-
[[Category: ZN]]
+
-
[[Category: folate hydrolase]]
+
-
[[Category: gcpii]]
+
-
[[Category: metallopeptidase]]
+
-
[[Category: naaladase]]
+
-
[[Category: prostate-specific membrane antigen]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 23:14:20 2007''
+

Current revision

A High Resolution Structure of Ligand-free Human Glutamate Carboxypeptidase II

PDB ID 2oot

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2oot"
Personal tools