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2orv

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(New page: 200px<br /> <applet load="2orv" size="450" color="white" frame="true" align="right" spinBox="true" caption="2orv, resolution 2.300&Aring;" /> '''human Thymidine Ki...)
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[[Image:2orv.gif|left|200px]]<br />
 
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<applet load="2orv" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2orv, resolution 2.300&Aring;" />
 
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'''human Thymidine Kinase 1 in complex with TP4A'''<br />
 
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==Overview==
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==human Thymidine Kinase 1 in complex with TP4A==
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Human thymidine kinase 1 (hTK1) and structurally related TKs from other, organisms catalyze the initial phosphorylation step in the thymidine, salvage pathway. Though ATP is known to be the preferred phosphoryl donor, for TK1-like enzymes, its exact binding mode and effect on the oligomeric, state has not been analyzed. Here we report the structures of hTK1 and of, the Thermotoga maritima thymidine kinase (TmTK) in complex with the, bisubstrate inhibitor TP4A. The TmTK-TP4A structure reveals that the, adenosine moiety of ATP binds at the subunit interface of the, homotetrameric enzyme and that the majority of the ATP-enzyme interactions, occur between the phosphate groups and the P-loop. In the hTK1 structure, the adenosine group of TP4A exhibited no electron density. This difference, between hTK1 and TmTK is rationalized by a difference in the conformation, of their quaternary structure. A more open conformation, as seen in the, TmTK-TP4A complex structure, is required to provide space for the, adenosine moiety. Our analysis supports the formation of an analogous open, conformation in hTK1 upon ATP binding.
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<StructureSection load='2orv' size='340' side='right'caption='[[2orv]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2orv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ORV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ORV FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4TA:P1-(5-ADENOSYL)P4-(5-(2-DEOXY-THYMIDYL))TETRAPHOSPHATE'>4TA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2orv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2orv OCA], [https://pdbe.org/2orv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2orv RCSB], [https://www.ebi.ac.uk/pdbsum/2orv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2orv ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/KITH_HUMAN KITH_HUMAN]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/or/2orv_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2orv ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2ORV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with ZN and 4TA as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Thymidine_kinase Thymidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.21 2.7.1.21] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2ORV OCA].
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*[[Thymidine kinase 3D structures|Thymidine kinase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Binding of ATP to TK1-like Enzymes Is Associated with a Conformational Change in the Quaternary Structure., Segura-Pena D, Lutz S, Monnerjahn C, Konrad M, Lavie A, J Mol Biol. 2007 May 25;369(1):129-41. Epub 2007 Mar 15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17407781 17407781]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Thymidine kinase]]
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[[Category: Konrad M]]
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[[Category: Konrad, M.]]
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[[Category: Lavie A]]
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[[Category: Lavie, A.]]
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[[Category: Lutz S]]
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[[Category: Lutz, S.]]
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[[Category: Monnerjahn C]]
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[[Category: Monnerjahn, C.]]
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[[Category: Segura-Pena D]]
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[[Category: Segura-Pena, D.]]
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[[Category: 4TA]]
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[[Category: ZN]]
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[[Category: tk-1 ( thymidine kinase 1)]]
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[[Category: tp4a (p1-(5'-adenosyl)p4-(5'-(2'deoxythymidil))tetraphosphate]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 23:15:26 2007''
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Current revision

human Thymidine Kinase 1 in complex with TP4A

PDB ID 2orv

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