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2p0e

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Human nicotinamide riboside kinase 1 in complex with tiazofurin

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Retrieved from "http://52.214.119.220/wiki/index.php/2p0e"

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-[[Image:2p0e.gif|left|200px]]<br />
-<applet load="2p0e" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="2p0e, resolution 1.800&Aring;" />
-'''Human nicotinamide riboside kinase 1 in complex with tiazofurin'''<br />
-==Overview==
+==Human nicotinamide riboside kinase 1 in complex with tiazofurin==
-The eukaryotic nicotinamide riboside kinase (Nrk) pathway, which is, induced in response to nerve damage and promotes replicative life span in, yeast, converts nicotinamide riboside to nicotinamide adenine dinucleotide, (NAD(+)) by phosphorylation and adenylylation. Crystal structures of human, Nrk1 bound to nucleoside and nucleotide substrates and products revealed, an enzyme structurally similar to Rossmann fold metabolite kinases and, allowed the identification of active site residues, which were shown to be, essential for human Nrk1 and Nrk2 activity in vivo. Although the, structures account for the 500-fold discrimination between nicotinamide, riboside and pyrimidine nucleosides, no enzyme feature was identified to, recognize the distinctive carboxamide group of nicotinamide riboside., Indeed, nicotinic acid riboside is a specific substrate of human Nrk, enzymes and is utilized in yeast in a novel biosynthetic pathway that, depends on Nrk and NAD(+) synthetase. Additionally, nicotinic acid, riboside is utilized in vivo by Urh1, Pnp1, and Preiss-Handler salvage., Thus, crystal structures of Nrk1 led to the identification of new pathways, to NAD(+).
+<StructureSection load='2p0e' size='340' side='right'caption='[[2p0e]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[2p0e]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P0E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2P0E FirstGlance]. <br>
-P0E is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with PO4, CL, TIZ and UNX as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2P0E OCA].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TIZ:(1R)-1-[4-(AMINOCARBONYL)-1,3-THIAZOL-2-YL]-1,4-ANHYDRO-D-RIBITOL'>TIZ</scene>, <scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene></td></tr>
-==Reference==
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2p0e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p0e OCA], [https://pdbe.org/2p0e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2p0e RCSB], [https://www.ebi.ac.uk/pdbsum/2p0e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2p0e ProSAT]</span></td></tr>
-Nicotinamide Riboside Kinase Structures Reveal New Pathways to NAD(+)., Tempel W, Rabeh WM, Bogan KL, Belenky P, Wojcik M, Seidle HF, Nedyalkova L, Yang T, Sauve AA, Park HW, Brenner C, PLoS Biol. 2007 Oct 2;5(10):e263. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17914902 17914902]
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/NRK1_HUMAN NRK1_HUMAN] Catalyzes the phosphorylation of nicotinamide riboside (NR) and nicotinic acid riboside (NaR) to form nicotinamide mononucleotide (NMN) and nicotinic acid mononucleotide (NaMN). The enzyme also phosphorylates the antitumor drugs tiazofurin and 3-deazaguanosine.<ref>PMID:15137942</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p0/2p0e_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2p0e ConSurf].
+<div style="clear:both"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Arrowsmith, C.H.]]
+[[Category: Arrowsmith CH]]
-[[Category: Bochkarev, A.]]
+[[Category: Bochkarev A]]
-[[Category: Brenner, C.]]
+[[Category: Brenner C]]
-[[Category: Edwards, A.M.]]
+[[Category: Edwards AM]]
-[[Category: Landry, R.]]
+[[Category: Landry R]]
-[[Category: Nedyalkova, L.]]
+[[Category: Nedyalkova L]]
-[[Category: Park, H.]]
+[[Category: Park H]]
-[[Category: Rabeh, W.M.]]
+[[Category: Rabeh WM]]
-[[Category: SGC, Structural.Genomics.Consortium.]]
+[[Category: Sundstrom M]]
-[[Category: Sundstrom, M.]]
+[[Category: Tempel W]]
-[[Category: Tempel, W.]]
+[[Category: Weigelt J]]
-[[Category: Weigelt, J.]]
-[[Category: CL]]
-[[Category: PO4]]
-[[Category: TIZ]]
-[[Category: UNX]]
-[[Category: adp]]
-[[Category: nad+]]
-[[Category: nicotinamide riboside]]
-[[Category: non-protein kinase]]
-[[Category: nrk1]]
-[[Category: sgc]]
-[[Category: structural genomics]]
-[[Category: structural genomics consortium]]
-[[Category: tiazofurin]]
-[[Category: transferase]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 23:19:01 2007''

2p0e

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Human nicotinamide riboside kinase 1 in complex with tiazofurin

Structural highlights

Function

Evolutionary Conservation

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