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2p82

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(New page: 200px<br /> <applet load="2p82" size="450" color="white" frame="true" align="right" spinBox="true" caption="2p82, resolution 2.10&Aring;" /> '''Cysteine protease A...)
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[[Image:2p82.gif|left|200px]]<br />
 
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<applet load="2p82" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2p82, resolution 2.10&Aring;" />
 
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'''Cysteine protease ATG4A'''<br />
 
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==Overview==
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==Cysteine protease ATG4A==
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We have cloned four human cDNAs encoding putative cysteine proteinases, that have been tentatively called autophagins. These proteins are similar, to Apg4/Aut2, a yeast enzyme involved in the activation of Apg8/Aut7, during the process of autophagy. The identified proteins ranging in length, from 393 to 474 amino acids also contain several structural features, characteristic of cysteine proteinases including a conserved cysteine, residue that is essential for the catalytic properties of these enzymes., Northern blot analysis demonstrated that autophagins are broadly, distributed in human tissues, being especially abundant in skeletal, muscle. Functional and morphological analysis in autophagy-defective yeast, strains lacking Apg4/Aut2 revealed that human autophagins-1 and -3 were, able to complement the deficiency in the yeast protease, restoring the, phenotypic and biochemical characteristics of autophagic cells. Enzymatic, studies performed with autophagin-3, the most widely expressed human, autophagin, revealed that the recombinant protein hydrolyzed the synthetic, substrate Mca-Thr-Phe-Gly-Met-Dpa-NH(2) whose sequence derives from that, present around the Apg4 cleavage site in yeast Apg8/Aut7. This proteolytic, activity was diminished by N-ethylmaleimide, an inhibitor of cysteine, proteases including yeast Apg4/Aut2. These results provide additional, evidence that the autophagic process widely studied in yeast can also be, fully reconstituted in human tissues and open the possibility to explore, the relevance of the autophagin-based proteolytic system in the induction, regulation, and execution of autophagy.
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<StructureSection load='2p82' size='340' side='right'caption='[[2p82]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2p82]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P82 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2P82 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2p82 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p82 OCA], [https://pdbe.org/2p82 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2p82 RCSB], [https://www.ebi.ac.uk/pdbsum/2p82 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2p82 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ATG4A_HUMAN ATG4A_HUMAN] Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP.<ref>PMID:15169837</ref> <ref>PMID:12473658</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p8/2p82_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2p82 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2P82 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with CL and EDO as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2P82 OCA].
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*[[Autophagy-related protein 3D structures|Autophagy-related protein 3D structures]]
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== References ==
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==Reference==
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<references/>
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Human autophagins, a family of cysteine proteinases potentially implicated in cell degradation by autophagy., Marino G, Uria JA, Puente XS, Quesada V, Bordallo J, Lopez-Otin C, J Biol Chem. 2003 Feb 7;278(6):3671-8. Epub 2002 Nov 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12446702 12446702]
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Arrowsmith, C.H.]]
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[[Category: Arrowsmith CH]]
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[[Category: Bochkarev, A.]]
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[[Category: Bochkarev A]]
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[[Category: Butler-Cole, C.]]
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[[Category: Butler-Cole C]]
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[[Category: Davis, T.]]
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[[Category: Davis T]]
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[[Category: Dhe-Paganon, S.]]
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[[Category: Dhe-Paganon S]]
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[[Category: Edwards, A.M.]]
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[[Category: Edwards AM]]
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[[Category: Jr., P.J.Finerty.]]
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[[Category: Finerty Jr PJ]]
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[[Category: Mujib, S.]]
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[[Category: Mujib S]]
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[[Category: SGC, Structural.Genomics.Consortium.]]
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[[Category: Sundstrom M]]
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[[Category: Sundstrom, M.]]
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[[Category: Walker JR]]
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[[Category: Walker, J.R.]]
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[[Category: Weigelt J]]
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[[Category: Weigelt, J.]]
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[[Category: CL]]
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[[Category: EDO]]
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[[Category: autophagy]]
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[[Category: hydrolase]]
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[[Category: protease]]
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[[Category: protein transport]]
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[[Category: sgc]]
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[[Category: structural genomics]]
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[[Category: structural genomics consortium]]
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[[Category: thiol protease]]
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[[Category: transport]]
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[[Category: ubl conjugation pathway]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 23:21:22 2007''
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Current revision

Cysteine protease ATG4A

PDB ID 2p82

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