2hoj

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[[Image:2hoj.gif|left|200px]]
 
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==Crystal structure of an E. coli thi-box riboswitch bound to thiamine pyrophosphate, manganese ions==
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The line below this paragraph, containing "STRUCTURE_2hoj", creates the "Structure Box" on the page.
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<StructureSection load='2hoj' size='340' side='right'caption='[[2hoj]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2hoj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HOJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HOJ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A23:ADENOSINE-5-PHOSPHATE-2,3-CYCLIC+PHOSPHATE'>A23</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=TPP:THIAMINE+DIPHOSPHATE'>TPP</scene></td></tr>
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{{STRUCTURE_2hoj| PDB=2hoj | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hoj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hoj OCA], [https://pdbe.org/2hoj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hoj RCSB], [https://www.ebi.ac.uk/pdbsum/2hoj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hoj ProSAT]</span></td></tr>
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</table>
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'''Crystal structure of an E. coli thi-box riboswitch bound to thiamine pyrophosphate, manganese ions'''
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==See Also==
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*[[Riboswitch 3D structures|Riboswitch 3D structures]]
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*[[Ribozyme 3D structures|Ribozyme 3D structures]]
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==Overview==
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__TOC__
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Riboswitches are noncoding mRNA elements that bind small-molecule metabolites with high affinity and specificity, and they regulate the expression of associated genes. The thi-box riboswitch can exhibit a 1000-fold higher affinity for thiamine pyrophosphate over closely related noncognate compounds such as thiamine monophosphate. To understand the chemical basis of thi-box pyrophosphate specificity, we have determined crystal structures of an E. coli thi-box bound to thiamine pyrophosphate, thiamine monophosphate, and the structural analogs benfotiamine and pyrithiamine. When bound to monophosphorylated compounds, the RNA elements that recognize the thiamine and phosphate moieties of the ligand move closer together. This allows the riboswitch to recognize the monophosphate in a manner similar to how it recognizes the beta-phosphate of thiamine pyrophosphate. In the pyrithiamine complex, the pyrophosphate binding site is largely unstructured. These results show how the riboswitch can bind to various metabolites, and why the thi-box preferentially binds thiamine pyrophosphate.
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</StructureSection>
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[[Category: Escherichia coli]]
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==About this Structure==
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[[Category: Large Structures]]
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HOJ OCA].
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[[Category: Edwards TE]]
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[[Category: Ferre-D'Amare AR]]
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==Reference==
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Crystal structures of the thi-box riboswitch bound to thiamine pyrophosphate analogs reveal adaptive RNA-small molecule recognition., Edwards TE, Ferre-D'Amare AR, Structure. 2006 Sep;14(9):1459-68. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16962976 16962976]
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[[Category: Amare, A R.Ferre-D.]]
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[[Category: Edwards, T E.]]
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[[Category: Riboswitch]]
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[[Category: Rna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:31:16 2008''
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Current revision

Crystal structure of an E. coli thi-box riboswitch bound to thiamine pyrophosphate, manganese ions

PDB ID 2hoj

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