2pon

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(New page: 200px<br /> <applet load="2pon" size="450" color="white" frame="true" align="right" spinBox="true" caption="2pon" /> '''Solution structure of the Bcl-xL/Beclin-1 c...)
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[[Image:2pon.gif|left|200px]]<br />
 
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<applet load="2pon" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2pon" />
 
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'''Solution structure of the Bcl-xL/Beclin-1 complex'''<br />
 
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==Overview==
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==Solution structure of the Bcl-xL/Beclin-1 complex==
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Beclin-1, originally identified as a Bcl-2 binding protein, is an, evolutionarily conserved protein required for autophagy. The direct, interaction between Beclin-1 and Bcl-2 or Bcl-xL provides a potential, convergence point for apoptosis and autophagy, two programmed cell death, processes. Given the functional significance of the interaction between, Beclin-1 and Bcl-2/Bcl-xL, we performed detailed biochemical and, structural characterizations of this interaction. We demonstrated that the, Bcl-xL-binding domain of Beclin-1 contains a BH3 domain. Therefore, Beclin-1 is a new member of the BH3-only family proteins. The structure of, Bcl-xL in complex with the Beclin-1 BH3 domain was determined at high, resolution by NMR spectroscopy. Although similar to other known BH3, domains, the Beclin-1 BH3 domain displays its own distinct features in the, complex with Bcl-xL. Systematic analysis of all known Bcl-xL/BH3 domain, complexes helped us to identify the molecular basis underlying the, capacity of Bcl-xL to recognize diverse target sequences.
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<StructureSection load='2pon' size='340' side='right'caption='[[2pon]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2pon]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PON OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PON FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pon FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pon OCA], [https://pdbe.org/2pon PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pon RCSB], [https://www.ebi.ac.uk/pdbsum/2pon PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pon ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/BECN1_HUMAN BECN1_HUMAN] Plays a central role in autophagy. Required for the abcission step in cytokinesis. May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.<ref>PMID:20208530</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/po/2pon_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pon ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Beclin-1, originally identified as a Bcl-2 binding protein, is an evolutionarily conserved protein required for autophagy. The direct interaction between Beclin-1 and Bcl-2 or Bcl-xL provides a potential convergence point for apoptosis and autophagy, two programmed cell death processes. Given the functional significance of the interaction between Beclin-1 and Bcl-2/Bcl-xL, we performed detailed biochemical and structural characterizations of this interaction. We demonstrated that the Bcl-xL-binding domain of Beclin-1 contains a BH3 domain. Therefore, Beclin-1 is a new member of the BH3-only family proteins. The structure of Bcl-xL in complex with the Beclin-1 BH3 domain was determined at high resolution by NMR spectroscopy. Although similar to other known BH3 domains, the Beclin-1 BH3 domain displays its own distinct features in the complex with Bcl-xL. Systematic analysis of all known Bcl-xL/BH3 domain complexes helped us to identify the molecular basis underlying the capacity of Bcl-xL to recognize diverse target sequences.
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==About this Structure==
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Molecular basis of Bcl-xL's target recognition versatility revealed by the structure of Bcl-xL in complex with the BH3 domain of Beclin-1.,Feng W, Huang S, Wu H, Zhang M J Mol Biol. 2007 Sep 7;372(1):223-35. Epub 2007 Jun 30. PMID:17659302<ref>PMID:17659302</ref>
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2PON is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2PON OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Molecular Basis of Bcl-xL's Target Recognition Versatility Revealed by the Structure of Bcl-xL in Complex with the BH3 Domain of Beclin-1., Feng W, Huang S, Wu H, Zhang M, J Mol Biol. 2007 Sep 7;372(1):223-35. Epub 2007 Jun 30. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17659302 17659302]
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</div>
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[[Category: Homo sapiens]]
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<div class="pdbe-citations 2pon" style="background-color:#fffaf0;"></div>
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[[Category: Protein complex]]
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[[Category: Feng, W.]]
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[[Category: Huang, S.]]
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[[Category: Wu, H.]]
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[[Category: Zhang, M.]]
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[[Category: apoptosis inhibitor]]
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[[Category: apoptosis; autophagy; bcl-2 family proteins; beclin-1]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 23:26:01 2007''
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==See Also==
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*[[B-cell lymphoma proteins 3D structures|B-cell lymphoma proteins 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Homo sapiens]]
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[[Category: Large Structures]]
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[[Category: Feng W]]
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[[Category: Huang S]]
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[[Category: Wu H]]
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[[Category: Zhang M]]

Current revision

Solution structure of the Bcl-xL/Beclin-1 complex

PDB ID 2pon

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Proteopedia Page Contributors and Editors (what is this?)

OCA

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