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| - | [[Image:2i2u.gif|left|200px]] | + | #REDIRECT [[4v50]] This PDB entry is obsolete and replaced by 4v50 |
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| - | The line below this paragraph, containing "STRUCTURE_2i2u", creates the "Structure Box" on the page.
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| - | {{STRUCTURE_2i2u| PDB=2i2u | SCENE= }}
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| - | '''Crystal Structure of Ribosome with messenger RNA and the Anticodon stem-loop of P-site tRNA. This file contains the 30s subunit of one 70s ribosome. The entire crystal structure contains two 70s ribosomes and is described in remark 400.'''
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| - | ==Overview==
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| - | Protein synthesis requires the accurate positioning of mRNA and tRNA in the peptidyl-tRNA site of the ribosome. Here we describe x-ray crystal structures of the intact bacterial ribosome from Escherichia coli in a complex with mRNA and the anticodon stem-loop of P-site tRNA. At 3.5-A resolution, these structures reveal rearrangements in the intact ribosome that clamp P-site tRNA and mRNA on the small ribosomal subunit. Binding of the anticodon stem-loop of P-site tRNA to the ribosome is sufficient to lock the head of the small ribosomal subunit in a single conformation, thereby preventing movement of mRNA and tRNA before mRNA decoding.
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| - | ==About this Structure==
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| - | 2I2U is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I2U OCA].
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| - | ==Reference==
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| - | Structural basis for mRNA and tRNA positioning on the ribosome., Berk V, Zhang W, Pai RD, Cate JH, Proc Natl Acad Sci U S A. 2006 Oct 24;103(43):15830-4. Epub 2006 Oct 12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17038497 17038497]
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| - | [[Category: Escherichia coli]]
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| - | [[Category: Protein complex]]
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| - | [[Category: Berk, V.]]
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| - | [[Category: Cate, J H.D.]]
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| - | [[Category: Pai, R D.]]
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| - | [[Category: Zhang, W.]]
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| - | [[Category: Protein-nucleic acid complex]]
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| - | [[Category: Ribosome]]
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:00:20 2008''
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