2i7e

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[[Image:2i7e.jpg|left|200px]]
 
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==GAAA tetralooop receptor complex with associated cobalt hexammine.==
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The line below this paragraph, containing "STRUCTURE_2i7e", creates the "Structure Box" on the page.
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<StructureSection load='2i7e' size='340' side='right'caption='[[2i7e]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2i7e]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I7E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I7E FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NCO:COBALT+HEXAMMINE(III)'>NCO</scene></td></tr>
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{{STRUCTURE_2i7e| PDB=2i7e | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i7e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i7e OCA], [https://pdbe.org/2i7e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i7e RCSB], [https://www.ebi.ac.uk/pdbsum/2i7e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i7e ProSAT]</span></td></tr>
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</table>
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'''GAAA tetralooop receptor complex with associated cobalt hexammine.'''
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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==Overview==
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Metal ions are critical for the proper folding of RNA, and the GAAA tetraloop-receptor is necessary for the optimal folding and function of many RNAs. We have used NMR to investigate the role of metal ions in the structure of the tetraloop-receptor in solution. The NMR data indicate native tertiary structure is formed under a wide range of ionic conditions. The lack of conformational adaptation in response to very different ionic conditions argues against a structural role for divalent ions. Nuclear Overhauser effects to cobalt hexammine and paramagnetic relaxation enhancement induced by manganese ions were used to determine the NMR structures of the tetraloop receptor in association with metal ions, providing the first atomic-level view of these interactions in the solution state. Five manganese and two cobalt hexammine ions could be localized to the RNA surface. The locations of the associated metal ions are similar, but not identical to, those of previously determined crystal structures. The sites of association are in general agreement with nonlinear Poisson-Boltzmann calculations of the electrostatic surface, emphasizing the general importance of diffusely associated ions in RNA tertiary structure.
Metal ions are critical for the proper folding of RNA, and the GAAA tetraloop-receptor is necessary for the optimal folding and function of many RNAs. We have used NMR to investigate the role of metal ions in the structure of the tetraloop-receptor in solution. The NMR data indicate native tertiary structure is formed under a wide range of ionic conditions. The lack of conformational adaptation in response to very different ionic conditions argues against a structural role for divalent ions. Nuclear Overhauser effects to cobalt hexammine and paramagnetic relaxation enhancement induced by manganese ions were used to determine the NMR structures of the tetraloop receptor in association with metal ions, providing the first atomic-level view of these interactions in the solution state. Five manganese and two cobalt hexammine ions could be localized to the RNA surface. The locations of the associated metal ions are similar, but not identical to, those of previously determined crystal structures. The sites of association are in general agreement with nonlinear Poisson-Boltzmann calculations of the electrostatic surface, emphasizing the general importance of diffusely associated ions in RNA tertiary structure.
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==About this Structure==
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Role of metal ions in the tetraloop-receptor complex as analyzed by NMR.,Davis JH, Foster TR, Tonelli M, Butcher SE RNA. 2007 Jan;13(1):76-86. Epub 2006 Nov 21. PMID:17119098<ref>PMID:17119098</ref>
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I7E OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Role of metal ions in the tetraloop-receptor complex as analyzed by NMR., Davis JH, Foster TR, Tonelli M, Butcher SE, RNA. 2007 Jan;13(1):76-86. Epub 2006 Nov 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17119098 17119098]
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</div>
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[[Category: Butcher, S E.]]
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<div class="pdbe-citations 2i7e" style="background-color:#fffaf0;"></div>
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[[Category: Davis, J H.]]
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== References ==
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[[Category: 11-nucleotide receptor]]
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<references/>
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[[Category: Gaaa tetraloop]]
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__TOC__
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[[Category: Rna tertiary structure]]
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</StructureSection>
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:10:25 2008''
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[[Category: Large Structures]]
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[[Category: Butcher SE]]
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[[Category: Davis JH]]

Current revision

GAAA tetralooop receptor complex with associated cobalt hexammine.

PDB ID 2i7e

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