2i8t

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[[Image:2i8t.jpg|left|200px]]
 
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==GDP-mannose mannosyl hydrolase-calcium-GDP-mannose complex==
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The line below this paragraph, containing "STRUCTURE_2i8t", creates the "Structure Box" on the page.
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<StructureSection load='2i8t' size='340' side='right'caption='[[2i8t]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2i8t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I8T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I8T FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GDD:GUANOSINE-5-DIPHOSPHATE-ALPHA-D-MANNOSE'>GDD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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{{STRUCTURE_2i8t| PDB=2i8t | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i8t OCA], [https://pdbe.org/2i8t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i8t RCSB], [https://www.ebi.ac.uk/pdbsum/2i8t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i8t ProSAT]</span></td></tr>
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</table>
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'''GDP-mannose mannosyl hydrolase-calcium-GDP-mannose complex'''
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== Function ==
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[https://www.uniprot.org/uniprot/Q6XQ58_ECOLX Q6XQ58_ECOLX] Hydrolyzes GDP-mannose.[HAMAP-Rule:MF_00941]
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Diversity in the polysaccharide component of lipopolysaccharide (LPS) contributes to the persistence and pathogenesis of Gram-negative bacteria. The Nudix hydrolase GDP-mannose mannosyl hydrolase (Gmm) contributes to this diversity by regulating the concentration of mannose in LPS biosynthetic pathways. Here, we present seven high-resolution crystal structures of Gmm from the enteropathogenic E. coli strain O128: the structure of the apo enzyme, the cocrystal structure of Gmm bound to the product Mg2+-GDP, two cocrystal structures of precatalytic and turnover complexes of Gmm-Ca2+-GDP-alpha-d-mannose, and three cocrystal structures of an inactive mutant (His-124 --&gt; Leu) Gmm bound to substrates GDP-alpha-d-mannose, GDP-alpha-d-glucose, and GDP-beta-l-fucose. These crystal structures help explain the molecular basis for substrate specificity and promiscuity and provide a structural framework for reconciling previously determined kinetic parameters. Unexpectedly, these structures reveal concerted changes in the enzyme structure that result in the formation of a catalytically competent active site only in the presence of the substrate/product. These structural views of the enzyme may provide a rationale for the design of inhibitors that target the biosynthesis of LPS by pathogenic bacteria.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i8/2i8t_consurf.spt"</scriptWhenChecked>
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2I8T is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I8T OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Molecular basis for substrate selectivity and specificity by an LPS biosynthetic enzyme., Zou Y, Li C, Brunzelle JS, Nair SK, Biochemistry. 2007 Apr 10;46(14):4294-304. Epub 2007 Mar 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17371001 17371001]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2i8t ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Brunzelle, J S.]]
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[[Category: Brunzelle JS]]
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[[Category: Li, C.]]
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[[Category: Li C]]
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[[Category: Nair, S K.]]
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[[Category: Nair SK]]
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[[Category: Zou, Y.]]
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[[Category: Zou Y]]
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[[Category: Lipopolysaccharide]]
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[[Category: Nudix enzyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:12:42 2008''
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Current revision

GDP-mannose mannosyl hydrolase-calcium-GDP-mannose complex

PDB ID 2i8t

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