2i9l

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[[Image:2i9l.jpg|left|200px]]
 
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==Structure of Fab 7D11 from a neutralizing antibody against the poxvirus L1 protein==
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The line below this paragraph, containing "STRUCTURE_2i9l", creates the "Structure Box" on the page.
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<StructureSection load='2i9l' size='340' side='right'caption='[[2i9l]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2i9l]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Vaccinia_virus Vaccinia virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I9L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I9L FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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{{STRUCTURE_2i9l| PDB=2i9l | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i9l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i9l OCA], [https://pdbe.org/2i9l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i9l RCSB], [https://www.ebi.ac.uk/pdbsum/2i9l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i9l ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PG095_VACCW PG095_VACCW] Component of the entry fusion complex (EFC), which consists of 11 proteins. During cell infection, this complex mediates entry of the virion core into the host cytoplasm by a two-step mechanism consisting of lipid mixing of the viral and cellular membranes and subsequent pore formation.<ref>PMID:34076488</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i9/2i9l_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2i9l ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Medical countermeasures to prevent or treat smallpox are needed due to the potential use of poxviruses as biological weapons. Safety concerns with the currently available smallpox vaccine indicate a need for research on alternative poxvirus vaccine strategies. Molecular vaccines involving the use of proteins and/or genes and recombinant antibodies are among the strategies under current investigation. The poxvirus L1 protein, encoded by the L1R open reading frame, is the target of neutralizing antibodies and has been successfully used as a component of both protein subunit and DNA vaccines. L1-specific monoclonal antibodies (e.g., mouse monoclonal antibody mAb-7D11, mAb-10F5) with potent neutralizing activity bind L1 in a conformation-specific manner. This suggests that proper folding of the L1 protein used in molecular vaccines will affect the production of neutralizing antibodies and protection. Here, we co-crystallized the Fab fragment of mAb-7D11 with the L1 protein. The crystal structure of the complex between Fab-7D11 and L1 reveals the basis for the conformation-specific binding as recognition of a discontinuous epitope containing two loops that are held together by a disulfide bond. The structure of this important conformational epitope of L1 will contribute to the development of molecular poxvirus vaccines and also provides a novel target for anti-poxvirus drugs. In addition, the sequence and structure of Fab-7D11 will contribute to the development of L1-targeted immunotherapeutics.
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'''Structure of Fab 7D11 from a neutralizing antibody against the poxvirus L1 protein'''
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Structural basis for the binding of the neutralizing antibody, 7D11, to the poxvirus L1 protein.,Su HP, Golden JW, Gittis AG, Hooper JW, Garboczi DN Virology. 2007 Nov 25;368(2):331-41. Epub 2007 Aug 3. PMID:17688903<ref>PMID:17688903</ref>
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==Overview==
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Medical countermeasures to prevent or treat smallpox are needed due to the potential use of poxviruses as biological weapons. Safety concerns with the currently available smallpox vaccine indicate a need for research on alternative poxvirus vaccine strategies. Molecular vaccines involving the use of proteins and/or genes and recombinant antibodies are among the strategies under current investigation. The poxvirus L1 protein, encoded by the L1R open reading frame, is the target of neutralizing antibodies and has been successfully used as a component of both protein subunit and DNA vaccines. L1-specific monoclonal antibodies (e.g., mouse monoclonal antibody mAb-7D11, mAb-10F5) with potent neutralizing activity bind L1 in a conformation-specific manner. This suggests that proper folding of the L1 protein used in molecular vaccines will affect the production of neutralizing antibodies and protection. Here, we co-crystallized the Fab fragment of mAb-7D11 with the L1 protein. The crystal structure of the complex between Fab-7D11 and L1 reveals the basis for the conformation-specific binding as recognition of a discontinuous epitope containing two loops that are held together by a disulfide bond. The structure of this important conformational epitope of L1 will contribute to the development of molecular poxvirus vaccines and also provides a novel target for anti-poxvirus drugs. In addition, the sequence and structure of Fab-7D11 will contribute to the development of L1-targeted immunotherapeutics.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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2I9L is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Vaccinia_virus Vaccinia virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I9L OCA].
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</div>
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<div class="pdbe-citations 2i9l" style="background-color:#fffaf0;"></div>
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==Reference==
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==See Also==
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Structural basis for the binding of the neutralizing antibody, 7D11, to the poxvirus L1 protein., Su HP, Golden JW, Gittis AG, Hooper JW, Garboczi DN, Virology. 2007 Nov 25;368(2):331-41. Epub 2007 Aug 3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17688903 17688903]
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*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Single protein]]
 
[[Category: Vaccinia virus]]
[[Category: Vaccinia virus]]
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[[Category: Garboczi, D N.]]
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[[Category: Garboczi DN]]
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[[Category: Gittis, A G.]]
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[[Category: Gittis AG]]
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[[Category: Golden, J W.]]
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[[Category: Golden JW]]
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[[Category: Hooper, J W.]]
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[[Category: Hooper JW]]
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[[Category: Moss, B.]]
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[[Category: Moss B]]
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[[Category: Su, H P.]]
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[[Category: Su HP]]
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[[Category: Antibody complex]]
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[[Category: Immune system/viral protein complex]]
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[[Category: Neutralizing antibody]]
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[[Category: Poxvirus]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:13:41 2008''
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Current revision

Structure of Fab 7D11 from a neutralizing antibody against the poxvirus L1 protein

PDB ID 2i9l

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