2if1

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[[Image:2if1.jpg|left|200px]]
 
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==HUMAN TRANSLATION INITIATION FACTOR EIF1, NMR, 29 STRUCTURES==
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The line below this paragraph, containing "STRUCTURE_2if1", creates the "Structure Box" on the page.
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<StructureSection load='2if1' size='340' side='right'caption='[[2if1]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2if1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IF1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IF1 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2if1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2if1 OCA], [https://pdbe.org/2if1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2if1 RCSB], [https://www.ebi.ac.uk/pdbsum/2if1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2if1 ProSAT]</span></td></tr>
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{{STRUCTURE_2if1| PDB=2if1 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/EIF1_HUMAN EIF1_HUMAN] Necessary for scanning and involved in initiation site selection. Promotes the assembly of 48S ribosomal complexes at the authentic initiation codon of a conventional capped mRNA.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/if/2if1_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2if1 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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eIF1 is a universally conserved translation factor that is necessary for scanning and involved in initiation site selection. We have determined the solution structure of human eIF1 with an N-terminal His tag using NMR spectroscopy. Residues 29-113 of the native sequence form a tightly packed domain with two alpha-helices on one side of a five-stranded parallel and antiparallel beta-sheet. The fold is new but similar to that of several ribosomal proteins and RNA-binding domains. A likely binding site is indicated by yeast mutations and conserved residues located together on the surface. No interaction with recombinant eIF5 or the initiation site RNA GCCACAAUGGCA was detected by NMR, but GST pull-down experiments show that eIF1 binds specifically to the p110 subunit of eIF3. This interaction explains how eIF1 is recruited to the 40S ribosomal subunit.
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'''HUMAN TRANSLATION INITIATION FACTOR EIF1, NMR, 29 STRUCTURES'''
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Structure and interactions of the translation initiation factor eIF1.,Fletcher CM, Pestova TV, Hellen CU, Wagner G EMBO J. 1999 May 4;18(9):2631-7. PMID:10228174<ref>PMID:10228174</ref>
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==Overview==
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eIF1 is a universally conserved translation factor that is necessary for scanning and involved in initiation site selection. We have determined the solution structure of human eIF1 with an N-terminal His tag using NMR spectroscopy. Residues 29-113 of the native sequence form a tightly packed domain with two alpha-helices on one side of a five-stranded parallel and antiparallel beta-sheet. The fold is new but similar to that of several ribosomal proteins and RNA-binding domains. A likely binding site is indicated by yeast mutations and conserved residues located together on the surface. No interaction with recombinant eIF5 or the initiation site RNA GCCACAAUGGCA was detected by NMR, but GST pull-down experiments show that eIF1 binds specifically to the p110 subunit of eIF3. This interaction explains how eIF1 is recruited to the 40S ribosomal subunit.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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2IF1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IF1 OCA].
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</div>
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<div class="pdbe-citations 2if1" style="background-color:#fffaf0;"></div>
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==Reference==
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==See Also==
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Structure and interactions of the translation initiation factor eIF1., Fletcher CM, Pestova TV, Hellen CU, Wagner G, EMBO J. 1999 May 4;18(9):2631-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10228174 10228174]
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*[[Eukaryotic initiation factor 3D structures|Eukaryotic initiation factor 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Fletcher, C M.]]
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[[Category: Fletcher CM]]
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[[Category: Hellen, C U.T.]]
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[[Category: Hellen CUT]]
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[[Category: Pestova, T V.]]
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[[Category: Pestova TV]]
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[[Category: Wagner, G.]]
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[[Category: Wagner G]]
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[[Category: Translation initiation factor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:25:46 2008''
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HUMAN TRANSLATION INITIATION FACTOR EIF1, NMR, 29 STRUCTURES

PDB ID 2if1

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