2iw1

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[[Image:2iw1.gif|left|200px]]
 
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==Crystal Structure of WaaG, a glycosyltransferase involved in lipopolysaccharide biosynthesis==
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The line below this paragraph, containing "STRUCTURE_2iw1", creates the "Structure Box" on the page.
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<StructureSection load='2iw1' size='340' side='right'caption='[[2iw1]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2iw1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_str._K-12_substr._W3110 Escherichia coli str. K-12 substr. W3110]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IW1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IW1 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=U2F:URIDINE-5-DIPHOSPHATE-2-DEOXY-2-FLUORO-ALPHA-D-GLUCOSE'>U2F</scene></td></tr>
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{{STRUCTURE_2iw1| PDB=2iw1 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2iw1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iw1 OCA], [https://pdbe.org/2iw1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2iw1 RCSB], [https://www.ebi.ac.uk/pdbsum/2iw1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2iw1 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RFAG_ECOLI RFAG_ECOLI] Involved in the addition of the first glucose residue to the lipopolysaccharide core.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/iw/2iw1_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2iw1 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Glycosyltransferases (GTs) catalyze the synthesis of the myriad glycoconjugates that are central to life. One of the largest families is GT4, which contains several enzymes of therapeutic significance, exemplified by WaaG and AviGT4. WaaG catalyses a key step in lipopolysaccharide synthesis, while AviGT4, produced by Streptomyces viridochromogenes, contributes to the synthesis of the antibiotic avilamycin A. Here we present the crystal structure of both WaaG and AviGT4. The two enzymes contain two "Rossmann-like" (beta/alpha/beta) domains characteristic of the GT-B fold. Both recognition of the donor substrate and the catalytic machinery is similar to other retaining GTs that display the GT-B fold. Structural information is discussed with respect to the evolution of GTs and the therapeutic significance of the two enzymes.
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'''CRYSTAL STRUCTURE OF WAAG, A GLYCOSYLTRANSFERASE INVOLVED IN LIPOPOLYSACCHARIDE BIOSYNTHESIS'''
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Insights into the synthesis of lipopolysaccharide and antibiotics through the structures of two retaining glycosyltransferases from family GT4.,Martinez-Fleites C, Proctor M, Roberts S, Bolam DN, Gilbert HJ, Davies GJ Chem Biol. 2006 Nov;13(11):1143-52. PMID:17113996<ref>PMID:17113996</ref>
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==Overview==
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Glycosyltransferases (GTs) catalyze the synthesis of the myriad glycoconjugates that are central to life. One of the largest families is GT4, which contains several enzymes of therapeutic significance, exemplified by WaaG and AviGT4. WaaG catalyses a key step in lipopolysaccharide synthesis, while AviGT4, produced by Streptomyces viridochromogenes, contributes to the synthesis of the antibiotic avilamycin A. Here we present the crystal structure of both WaaG and AviGT4. The two enzymes contain two "Rossmann-like" (beta/alpha/beta) domains characteristic of the GT-B fold. Both recognition of the donor substrate and the catalytic machinery is similar to other retaining GTs that display the GT-B fold. Structural information is discussed with respect to the evolution of GTs and the therapeutic significance of the two enzymes.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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2IW1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IW1 OCA].
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</div>
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<div class="pdbe-citations 2iw1" style="background-color:#fffaf0;"></div>
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==Reference==
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==See Also==
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Insights into the synthesis of lipopolysaccharide and antibiotics through the structures of two retaining glycosyltransferases from family GT4., Martinez-Fleites C, Proctor M, Roberts S, Bolam DN, Gilbert HJ, Davies GJ, Chem Biol. 2006 Nov;13(11):1143-52. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17113996 17113996]
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*[[Glycosyltransferase 3D structures|Glycosyltransferase 3D structures]]
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[[Category: Escherichia coli]]
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== References ==
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[[Category: Single protein]]
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<references/>
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[[Category: Bolam, D N.]]
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__TOC__
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[[Category: Davies, G J.]]
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</StructureSection>
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[[Category: Gilbert, H J.]]
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[[Category: Escherichia coli str. K-12 substr. W3110]]
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[[Category: Martinez-Fleites, C.]]
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[[Category: Large Structures]]
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[[Category: Proctor, M.]]
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[[Category: Bolam DN]]
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[[Category: Roberts, S.]]
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[[Category: Davies GJ]]
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[[Category: Family gt-4]]
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[[Category: Gilbert HJ]]
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[[Category: Glycosyltransferase]]
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[[Category: Martinez-Fleites C]]
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[[Category: Lipopolysaccharide biosynthesis]]
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[[Category: Proctor M]]
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[[Category: Lp]]
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[[Category: Roberts S]]
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[[Category: Retaining]]
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[[Category: Transferase]]
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[[Category: Udp-glucose]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:57:45 2008''
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Current revision

Crystal Structure of WaaG, a glycosyltransferase involved in lipopolysaccharide biosynthesis

PDB ID 2iw1

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