2v9y

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[[Image:2v9y.gif|left|200px]]<br />
 
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<applet load="2v9y" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2v9y, resolution 2.10&Aring;" />
 
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'''HUMAN AMINOIMIDAZOLE RIBONUCLEOTIDE SYNTHETASE'''<br />
 
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==Overview==
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==Human aminoimidazole ribonucleotide synthetase==
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BACKGROUND: The purine biosynthetic pathway in procaryotes enlists eleven, enzymes, six of which use ATP. Enzymes 5 and 6 of this pathway, formylglycinamide ribonucleotide (FGAR) amidotransferase (PurL) and, aminoimidazole ribonucleotide (AIR) synthetase (PurM) utilize ATP to, activate the oxygen of an amide within their substrate toward nucleophilic, attack by a nitrogen. AIR synthetase uses the product of PurL, formylglycinamidine ribonucleotide (FGAM) and ATP to make AIR, ADP and, P(i). RESULTS: The structure of a hexahistidine-tagged PurM has been, solved by multiwavelength anomalous diffraction phasing techniques using, protein containing 28 selenomethionines per asymmetric unit. The final, model of PurM consists of two crystallographically independent dimers and, four sulfates. The overall R factor at 2.5 A resolution is 19.2%, with an, R(free) of 26.4%. The active site, identified in part by conserved, residues, is proposed to be a long groove generated by the interaction of, two monomers. A search of the sequence databases suggests that the, ATP-binding sites between PurM and PurL may be structurally conserved., CONCLUSIONS: The first structure of a new class of ATP-binding enzyme, PurM, has been solved and a model for the active site has been proposed., The structure is unprecedented, with an extensive and unusual, sheet-mediated intersubunit interaction defining the active-site grooves., Sequence searches suggest that two successive enzymes in the purine, biosynthetic pathway, proposed to use similar chemistries, will have, similar ATP-binding domains.
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<StructureSection load='2v9y' size='340' side='right'caption='[[2v9y]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2v9y]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V9Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2V9Y FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2v9y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v9y OCA], [https://pdbe.org/2v9y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2v9y RCSB], [https://www.ebi.ac.uk/pdbsum/2v9y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2v9y ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PUR2_HUMAN PUR2_HUMAN]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v9/2v9y_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2v9y ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Human purine de novo synthesis pathway contains several multi-functional enzymes, one of which, tri-functional GART, contains three enzymatic activities in a single polypeptide chain. We have solved structures of two domains bearing separate catalytic functions: glycinamide ribonucleotide synthetase and aminoimidazole ribonucleotide synthetase. Structures are compared with those of homologous enzymes from prokaryotes and analyzed in terms of the catalytic mechanism. We also report small angle X-ray scattering models for the full-length protein. These models are consistent with the enzyme forming a dimer through the middle domain. The protein has an approximate seesaw geometry where terminal enzyme units display high mobility owing to flexible linker segments. This resilient seesaw shape may facilitate internal substrate/product transfer or forwarding to other enzymes in the pathway.
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==About this Structure==
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Structural studies of tri-functional human GART.,Welin M, Grossmann JG, Flodin S, Nyman T, Stenmark P, Tresaugues L, Kotenyova T, Johansson I, Nordlund P, Lehtio L Nucleic Acids Res. 2010 Nov 1;38(20):7308-19. Epub 2010 Jul 14. PMID:20631005<ref>PMID:20631005</ref>
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2V9Y is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Phosphoribosylformylglycinamidine_cyclo-ligase Phosphoribosylformylglycinamidine cyclo-ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.3.1 6.3.3.1] Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2V9Y OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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X-ray crystal structure of aminoimidazole ribonucleotide synthetase (PurM), from the Escherichia coli purine biosynthetic pathway at 2.5 A resolution., Li C, Kappock TJ, Stubbe J, Weaver TM, Ealick SE, Structure. 1999 Sep 15;7(9):1155-66. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10508786 10508786]
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</div>
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<div class="pdbe-citations 2v9y" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Phosphoribosylformylglycinamidine cyclo-ligase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Arrowsmith CH]]
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[[Category: Arrowsmith, C.H.]]
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[[Category: Berglund H]]
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[[Category: Berg, S.Van.Den.]]
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[[Category: Busam R]]
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[[Category: Berglund, H.]]
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[[Category: Collins R]]
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[[Category: Busam, R.]]
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[[Category: Dahlgren LG]]
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[[Category: Collins, R.]]
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[[Category: Edwards AM]]
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[[Category: Consortium, Structural.Genomics.]]
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[[Category: Flodin S]]
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[[Category: Dahlgren, L.G.]]
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[[Category: Flores A]]
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[[Category: Edwards, A.]]
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[[Category: Graslund S]]
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[[Category: Flodin, S.]]
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[[Category: Hallberg BM]]
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[[Category: Flores, A.]]
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[[Category: Hammarstrom M]]
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[[Category: Graslund, S.]]
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[[Category: Herman MD]]
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[[Category: Hallberg, B.M.]]
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[[Category: Holmberg-Schiavone L]]
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[[Category: Hammarstrom, M.]]
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[[Category: Johansson I]]
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[[Category: Herman, M.D.]]
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[[Category: Kallas A]]
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[[Category: Holmberg-Schiavone, L.]]
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[[Category: Karlberg T]]
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[[Category: Johansson, I.]]
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[[Category: Kotenyova T]]
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[[Category: Kallas, A.]]
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[[Category: Lehtio L]]
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[[Category: Karlberg, T.]]
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[[Category: Moche M]]
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[[Category: Kotenyova, T.]]
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[[Category: Nordlund P]]
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[[Category: Lehtio, L.]]
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[[Category: Nyman T]]
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[[Category: Moche, M.]]
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[[Category: Persson C]]
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[[Category: Nordlund, P.]]
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[[Category: Sagemark J]]
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[[Category: Nyman, T.]]
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[[Category: Stenmark P]]
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[[Category: Persson, C.]]
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[[Category: Sundstrom M]]
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[[Category: Sagemark, J.]]
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[[Category: Thorsell AG]]
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[[Category: Stenmark, P.]]
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[[Category: Tresaugues L]]
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[[Category: Sundstrom, M.]]
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[[Category: Weigelt J]]
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[[Category: Thorsell, A.G.]]
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[[Category: Welin M]]
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[[Category: Tresaugues, L.]]
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[[Category: Van den Berg S]]
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[[Category: Weigelt, J.]]
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[[Category: Welin, M.]]
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[[Category: SO4]]
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[[Category: airs]]
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[[Category: alternative splicing]]
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[[Category: aminoimidazole ribonucleotide synthetase]]
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[[Category: atp-binding]]
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[[Category: gart]]
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[[Category: ligase]]
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[[Category: multifunctional enzyme]]
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[[Category: nucleotide-binding]]
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[[Category: phosphorylation]]
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[[Category: polymorphism]]
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[[Category: purine biosynthesis]]
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[[Category: purine metabolism]]
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[[Category: sgc]]
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[[Category: structural genomics]]
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[[Category: structural genomics consortium]]
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[[Category: transferase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 23:42:54 2007''
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Current revision

Human aminoimidazole ribonucleotide synthetase

PDB ID 2v9y

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