2j85
From Proteopedia
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| - | [[Image:2j85.gif|left|200px]] | ||
| - | + | ==B116 of Sulfolobus turreted icosahedral virus (STIV)== | |
| - | + | <StructureSection load='2j85' size='340' side='right'caption='[[2j85]], [[Resolution|resolution]] 2.39Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[2j85]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfolobus_turreted_icosahedral_virus Sulfolobus turreted icosahedral virus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2blk 2blk]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J85 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J85 FirstGlance]. <br> | |
| - | + | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | |
| - | --> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j85 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j85 OCA], [https://pdbe.org/2j85 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j85 RCSB], [https://www.ebi.ac.uk/pdbsum/2j85 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j85 ProSAT]</span></td></tr> |
| - | + | </table> | |
| - | + | == Evolutionary Conservation == | |
| - | ''' | + | [[Image:Consurf_key_small.gif|200px|right]] |
| - | + | Check<jmol> | |
| - | + | <jmolCheckbox> | |
| - | == | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j8/2j85_consurf.spt"</scriptWhenChecked> |
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2j85 ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
Sulfolobus turreted icosahedral virus (STIV) infects Sulfolobus species found in the hot springs of Yellowstone National Park. Its 37 open reading frames (ORFs) generally lack sequence similarity to other genes. One exception, however, is ORF B116. While its function is unknown, orthologs are found in three additional crenarchaeal viral families. Due to the central importance of this protein family to crenarchaeal viruses, we have undertaken structural and biochemical studies of B116. The structure reveals a previously unobserved fold consisting of a five-stranded beta-sheet flanked on one side by three alpha helices. Two subunits come together to form a homodimer with a 10-stranded mixed beta-sheet, where the topology of the central strands resembles an unclosed beta-barrel. Highly conserved loops rise above the surface of the saddle-shaped protein and suggest an interaction with the major groove of DNA. The predicted B116-DNA interaction is confirmed by electrophoretic mobility shift assays. | Sulfolobus turreted icosahedral virus (STIV) infects Sulfolobus species found in the hot springs of Yellowstone National Park. Its 37 open reading frames (ORFs) generally lack sequence similarity to other genes. One exception, however, is ORF B116. While its function is unknown, orthologs are found in three additional crenarchaeal viral families. Due to the central importance of this protein family to crenarchaeal viruses, we have undertaken structural and biochemical studies of B116. The structure reveals a previously unobserved fold consisting of a five-stranded beta-sheet flanked on one side by three alpha helices. Two subunits come together to form a homodimer with a 10-stranded mixed beta-sheet, where the topology of the central strands resembles an unclosed beta-barrel. Highly conserved loops rise above the surface of the saddle-shaped protein and suggest an interaction with the major groove of DNA. The predicted B116-DNA interaction is confirmed by electrophoretic mobility shift assays. | ||
| - | + | A new DNA binding protein highly conserved in diverse crenarchaeal viruses.,Larson ET, Eilers BJ, Reiter D, Ortmann AC, Young MJ, Lawrence CM Virology. 2007 Jul 5;363(2):387-96. Epub 2007 Mar 2. PMID:17336360<ref>PMID:17336360</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | [[Category: | + | <div class="pdbe-citations 2j85" style="background-color:#fffaf0;"></div> |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
[[Category: Sulfolobus turreted icosahedral virus]] | [[Category: Sulfolobus turreted icosahedral virus]] | ||
| - | [[Category: Larson, E T | + | [[Category: Larson, E T]] |
| - | [[Category: Lawrence, C M | + | [[Category: Lawrence, C M]] |
| - | [[Category: Reiter, D | + | [[Category: Reiter, D]] |
| - | [[Category: Young, M J | + | [[Category: Young, M J]] |
[[Category: Archaea]] | [[Category: Archaea]] | ||
[[Category: Archaeal virus]] | [[Category: Archaeal virus]] | ||
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[[Category: Stiv]] | [[Category: Stiv]] | ||
[[Category: Sulfolobus]] | [[Category: Sulfolobus]] | ||
| - | [[Category: Sulfolobus turreted icosahedral virus]] | ||
[[Category: Viral protein]] | [[Category: Viral protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 08:29:15 2008'' | ||
Current revision
B116 of Sulfolobus turreted icosahedral virus (STIV)
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