2z65

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(New page: 200px<br /> <applet load="2z65" size="450" color="white" frame="true" align="right" spinBox="true" caption="2z65, resolution 2.70&Aring;" /> '''Crystal structure o...)
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[[Image:2z65.gif|left|200px]]<br />
 
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<applet load="2z65" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2z65, resolution 2.70&Aring;" />
 
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'''Crystal structure of the human TLR4 TV3 hybrid-MD-2-Eritoran complex'''<br />
 
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==Overview==
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==Crystal structure of the human TLR4 TV3 hybrid-MD-2-Eritoran complex==
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TLR4 and MD-2 form a heterodimer that recognizes LPS (lipopolysaccharide), from Gram-negative bacteria. Eritoran is an analog of LPS that antagonizes, its activity by binding to the TLR4-MD-2 complex. We determined the, structure of the full-length ectodomain of the mouse TLR4 and MD-2, complex. We also produced a series of hybrids of human TLR4 and hagfish, VLR and determined their structures with and without bound MD-2 and, Eritoran. TLR4 is an atypical member of the LRR family and is composed of, N-terminal, central, and C-terminal domains. The beta sheet of the central, domain shows unusually small radii and large twist angles. MD-2 binds to, the concave surface of the N-terminal and central domains. The interaction, with Eritoran is mediated by a hydrophobic internal pocket in MD-2. Based, on structural analysis and mutagenesis experiments on MD-2 and TLR4, we, propose a model of TLR4-MD-2 dimerization induced by LPS.
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<StructureSection load='2z65' size='340' side='right'caption='[[2z65]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2z65]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Eptatretus_burgeri Eptatretus burgeri] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z65 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Z65 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=E55:3-O-DECYL-2-DEOXY-6-O-{2-DEOXY-3-O-[(3R)-3-METHOXYDECYL]-6-O-METHYL-2-[(11Z)-OCTADEC-11-ENOYLAMINO]-4-O-PHOSPHONO-BETA-D-GLUCOPYRANOSYL}-2-[(3-OXOTETRADECANOYL)AMINO]-1-O-PHOSPHONO-ALPHA-D-GLUCOPYRANOSE'>E55</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2z65 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z65 OCA], [https://pdbe.org/2z65 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2z65 RCSB], [https://www.ebi.ac.uk/pdbsum/2z65 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2z65 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LY96_HUMAN LY96_HUMAN] Cooperates with TLR4 in the innate immune response to bacterial lipopolysaccharide (LPS), and with TLR2 in the response to cell wall components from Gram-positive and Gram-negative bacteria. Enhances TLR4-dependent activation of NF-kappa-B. Cells expressing both MD2 and TLR4, but not TLR4 alone, respond to LPS.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z6/2z65_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2z65 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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TLR4 and MD-2 form a heterodimer that recognizes LPS (lipopolysaccharide) from Gram-negative bacteria. Eritoran is an analog of LPS that antagonizes its activity by binding to the TLR4-MD-2 complex. We determined the structure of the full-length ectodomain of the mouse TLR4 and MD-2 complex. We also produced a series of hybrids of human TLR4 and hagfish VLR and determined their structures with and without bound MD-2 and Eritoran. TLR4 is an atypical member of the LRR family and is composed of N-terminal, central, and C-terminal domains. The beta sheet of the central domain shows unusually small radii and large twist angles. MD-2 binds to the concave surface of the N-terminal and central domains. The interaction with Eritoran is mediated by a hydrophobic internal pocket in MD-2. Based on structural analysis and mutagenesis experiments on MD-2 and TLR4, we propose a model of TLR4-MD-2 dimerization induced by LPS.
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==About this Structure==
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Crystal structure of the TLR4-MD-2 complex with bound endotoxin antagonist Eritoran.,Kim HM, Park BS, Kim JI, Kim SE, Lee J, Oh SC, Enkhbayar P, Matsushima N, Lee H, Yoo OJ, Lee JO Cell. 2007 Sep 7;130(5):906-17. PMID:17803912<ref>PMID:17803912</ref>
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2Z65 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Homo_sapiens,_eptatretus_burgeri Homo sapiens, eptatretus burgeri] with NAG, NDG and E55 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2Z65 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal Structure of the TLR4-MD-2 Complex with Bound Endotoxin Antagonist Eritoran., Kim HM, Park BS, Kim JI, Kim SE, Lee J, Oh SC, Enkhbayar P, Matsushima N, Lee H, Yoo OJ, Lee JO, Cell. 2007 Sep 7;130(5):906-17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17803912 17803912]
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</div>
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[[Category: Homo sapiens]]
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<div class="pdbe-citations 2z65" style="background-color:#fffaf0;"></div>
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[[Category: Homo sapiens, eptatretus burgeri]]
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[[Category: Protein complex]]
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[[Category: Kim, H.M.]]
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[[Category: Lee, J.O.]]
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[[Category: Park, B.S.]]
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[[Category: E55]]
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[[Category: NAG]]
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[[Category: NDG]]
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[[Category: e5564]]
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[[Category: eritoran]]
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[[Category: glycoprotein]]
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[[Category: immune response]]
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[[Category: immune system]]
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[[Category: inflammatory response]]
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[[Category: innate immunity]]
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[[Category: leucine-rich repeat]]
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[[Category: lps]]
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[[Category: md-2]]
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[[Category: membrane]]
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[[Category: polymorphism]]
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[[Category: secreted]]
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[[Category: tlr4]]
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[[Category: toll-like receptor]]
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[[Category: transmembrane]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 23:47:19 2007''
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==See Also==
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*[[Toll-like Receptor 3D structures|Toll-like Receptor 3D structures]]
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*[[Variable lymphocyte receptor 3D structures|Variable lymphocyte receptor 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Eptatretus burgeri]]
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[[Category: Homo sapiens]]
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[[Category: Large Structures]]
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[[Category: Kim HM]]
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[[Category: Lee J-O]]
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[[Category: Park BS]]

Current revision

Crystal structure of the human TLR4 TV3 hybrid-MD-2-Eritoran complex

PDB ID 2z65

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