2z6o

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /> <applet load="2z6o" size="450" color="white" frame="true" align="right" spinBox="true" caption="2z6o, resolution 1.60&Aring;" /> '''Crystal Structure o...)
Current revision (13:23, 1 November 2023) (edit) (undo)
 
(16 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2z6o.gif|left|200px]]<br />
 
-
<applet load="2z6o" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="2z6o, resolution 1.60&Aring;" />
 
-
'''Crystal Structure of the Ufc1, Ufm1 conjugating enzyme 1'''<br />
 
-
==Overview==
+
==Crystal Structure of the Ufc1, Ufm1 conjugating enzyme 1==
-
Ubiquitin and ubiquitin-like protein-conjugating enzymes play central, roles in posttranslational modification processes. The ubiquitin-fold, modifier 1 (Ufm1), one of a variety of ubiquitin-like modifiers, is, covalently attached to target proteins via Uba5 and Ufm1-conjugating, enzyme 1 (Ufc1), which are analogous to the E1 and E2 ubiquitylation, enzymes. As Ufm1-related proteins are conserved in metazoa and plants, the, Ufm1 system likely plays important roles in various multicellular, organisms. Herein, we report the X-ray structure of human Ufc1 determined, at 1.6A resolution. The Ufc1 structure comprises a canonical E2 domain and, an additional N-terminal domain. The Uba5 binding site on Ufc1 was, assigned by structural comparison of Ufc1 and Ubc12 and related mutational, analyses. In addition, we show that the N-terminal unique domain of Ufc1, contributes to thermal stability.
+
<StructureSection load='2z6o' size='340' side='right'caption='[[2z6o]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2z6o]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z6O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Z6O FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2z6o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z6o OCA], [https://pdbe.org/2z6o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2z6o RCSB], [https://www.ebi.ac.uk/pdbsum/2z6o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2z6o ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/UFC1_HUMAN UFC1_HUMAN] E2-like enzyme which forms an intermediate with UFM1 via a thioester linkage.<ref>PMID:15071506</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z6/2z6o_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2z6o ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Ubiquitin and ubiquitin-like protein-conjugating enzymes play central roles in posttranslational modification processes. The ubiquitin-fold modifier 1 (Ufm1), one of a variety of ubiquitin-like modifiers, is covalently attached to target proteins via Uba5 and Ufm1-conjugating enzyme 1 (Ufc1), which are analogous to the E1 and E2 ubiquitylation enzymes. As Ufm1-related proteins are conserved in metazoa and plants, the Ufm1 system likely plays important roles in various multicellular organisms. Herein, we report the X-ray structure of human Ufc1 determined at 1.6 A resolution. The Ufc1 structure comprises a canonical E2 domain and an additional N-terminal domain. The Uba5 binding site on Ufc1 was assigned by structural comparison of Ufc1 and Ubc12 and related mutational analyses. In addition, we show that the N-terminal unique domain of Ufc1 contributes to thermal stability.
-
==About this Structure==
+
Crystal structure of Ufc1, the Ufm1-conjugating enzyme.,Mizushima T, Tatsumi K, Ozaki Y, Kawakami T, Suzuki A, Ogasahara K, Komatsu M, Kominami E, Tanaka K, Yamane T Biochem Biophys Res Commun. 2007 Nov 3;362(4):1079-84. Epub 2007 Aug 30. PMID:17825256<ref>PMID:17825256</ref>
-
2Z6O is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2Z6O OCA].
+
-
==Reference==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
Crystal structure of Ufc1, the Ufm1-conjugating enzyme., Mizushima T, Tatsumi K, Ozaki Y, Kawakami T, Suzuki A, Ogasahara K, Komatsu M, Kominami E, Tanaka K, Yamane T, Biochem Biophys Res Commun. 2007 Nov 3;362(4):1079-84. Epub 2007 Aug 30. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17825256 17825256]
+
</div>
-
[[Category: Homo sapiens]]
+
<div class="pdbe-citations 2z6o" style="background-color:#fffaf0;"></div>
-
[[Category: Single protein]]
+
-
[[Category: Ubiquitin--protein ligase]]
+
-
[[Category: Kawakami, T.]]
+
-
[[Category: Komatsu, M.]]
+
-
[[Category: Kominami, E.]]
+
-
[[Category: Mizushima ,T.]]
+
-
[[Category: Ogasahara, K.]]
+
-
[[Category: Ozaki, Y.]]
+
-
[[Category: Suzuki, A.]]
+
-
[[Category: Tanaka, K.]]
+
-
[[Category: Tatsumi, K.]]
+
-
[[Category: Yamane, T.]]
+
-
[[Category: MG]]
+
-
[[Category: ligase]]
+
-
[[Category: polymorphism]]
+
-
[[Category: ubiquitin]]
+
-
[[Category: ubl]]
+
-
[[Category: ubl conjugation pathway]]
+
-
[[Category: ufc1]]
+
-
[[Category: ufm1]]
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 23:47:23 2007''
+
==See Also==
 +
*[[Ferritin 3D structures|Ferritin 3D structures]]
 +
*[[Ubiquitin-fold modifier|Ubiquitin-fold modifier]]
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Homo sapiens]]
 +
[[Category: Large Structures]]
 +
[[Category: Kawakami T]]
 +
[[Category: Komatsu M]]
 +
[[Category: Kominami E]]
 +
[[Category: Mizushima T]]
 +
[[Category: Ogasahara K]]
 +
[[Category: Ozaki Y]]
 +
[[Category: Suzuki A]]
 +
[[Category: Tanaka K]]
 +
[[Category: Tatsumi K]]
 +
[[Category: Yamane T]]

Current revision

Crystal Structure of the Ufc1, Ufm1 conjugating enzyme 1

PDB ID 2z6o

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2z6o"
Personal tools