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2jvr

From Proteopedia

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[[Image:2jvr.jpg|left|200px]]
 
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==Segmental Isotope Labeling of Npl3p==
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The line below this paragraph, containing "STRUCTURE_2jvr", creates the "Structure Box" on the page.
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<StructureSection load='2jvr' size='340' side='right'caption='[[2jvr]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2jvr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JVR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JVR FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jvr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jvr OCA], [https://pdbe.org/2jvr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jvr RCSB], [https://www.ebi.ac.uk/pdbsum/2jvr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jvr ProSAT]</span></td></tr>
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{{STRUCTURE_2jvr| PDB=2jvr | SCENE= }}
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</table>
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== Function ==
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'''Segmental Isotope Labeling of Npl3p'''
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[https://www.uniprot.org/uniprot/NOP3_YEAST NOP3_YEAST] Required for pre-rRNA processing and nuclear import as well as mitochondrial protein targeting. Binds to poly(A).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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The study of multidomain or large proteins in solution by NMR spectroscopy has been made possible in recent years by the development of new spectroscopic methods. However, resonance overlap found in large proteins remains a limiting factor, making resonance assignments and structure determination of large proteins very difficult. In this study, we present an expressed protein ligation protocol that can be used for the segmental isotopic labeling of virtually any multidomain or high molecular mass protein, independent of both the folding state and the solubility of the protein fragments, as well as independent of whether the fragments are interacting. The protocol was applied successfully to two different multidomain proteins containing RNA recognition motifs (RRMs), heterogeneous nuclear ribonucleoprotein L and Npl3p. High yields of segmentally labeled proteins could be obtained, allowing characterization of the interdomain interactions with NMR spectroscopy. We found that the RRMs of heterogeneous nuclear ribonucleoprotein L interact, whereas those of Npl3p are independent. Subsequently, the structures of the two RRMs of Npl3p were determined on the basis of samples in which each RRM was expressed individually. The two Npl3p RRMs adopt the expected beta alpha beta beta alpha beta fold.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jv/2jvr_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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2JVR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JVR OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2jvr ConSurf].
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Improved segmental isotope labeling methods for the NMR study of multidomain or large proteins: application to the RRMs of Npl3p and hnRNP L., Skrisovska L, Allain FH, J Mol Biol. 2008 Jan 4;375(1):151-64. Epub 2007 Sep 16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17936301 17936301]
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Allain FH-T]]
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[[Category: Allain, F H-T.]]
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[[Category: Skrisovska L]]
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[[Category: Skrisovska, L.]]
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[[Category: Nucleus]]
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[[Category: Phosphorylation]]
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[[Category: Protein]]
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[[Category: Ribonucleoprotein]]
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[[Category: Ribosome biogenesis]]
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[[Category: Rna binding protein]]
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[[Category: Rna recognition motif]]
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[[Category: Rna-binding]]
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[[Category: Rrna processing]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:20:23 2008''
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Current revision

Segmental Isotope Labeling of Npl3p

PDB ID 2jvr

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