|
m |
| (3 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| - | [[Image:2mm1.gif|left|200px]] | + | #REDIRECT [[3rgk]] This PDB entry is obsolete and replaced by 3rgk |
| - | | + | |
| - | <!--
| + | |
| - | The line below this paragraph, containing "STRUCTURE_2mm1", creates the "Structure Box" on the page.
| + | |
| - | You may change the PDB parameter (which sets the PDB file loaded into the applet)
| + | |
| - | or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| + | |
| - | or leave the SCENE parameter empty for the default display.
| + | |
| - | -->
| + | |
| - | {{STRUCTURE_2mm1| PDB=2mm1 | SCENE= }}
| + | |
| - | | + | |
| - | '''X-RAY CRYSTAL STRUCTURE OF A RECOMBINANT HUMAN MYOGLOBIN MUTANT AT 2.8 ANGSTROMS RESOLUTION'''
| + | |
| - | | + | |
| - | | + | |
| - | ==Overview==
| + | |
| - | We have grown crystals in trigonal space group P3(2)21 of a mutant human myoglobin, aquomet form, in which lysine at position 45 has been replaced by arginine and cysteine at position 110 has been replaced by alanine. Suitable crystals of native recombinant human myoglobin have not been obtained. We have used the molecular replacement method to determine the X-ray crystal structure of the mutant at 2.8 A resolution. At the present stage of refinement, the crystallographic R-value for the model, with tightly restrained stereochemistry, is 0.158 for 5.0 to 2.8 A data. As expected, the overall structure is quite similar to the sperm whale myoglobin structure. Arginine 45 adopts a well-ordered conformation similar to that found in aquomet sperm whale myoglobin.
| + | |
| - | | + | |
| - | ==About this Structure==
| + | |
| - | 2MM1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MM1 OCA].
| + | |
| - | | + | |
| - | ==Reference==
| + | |
| - | X-ray crystal structure of a recombinant human myoglobin mutant at 2.8 A resolution., Hubbard SR, Hendrickson WA, Lambright DG, Boxer SG, J Mol Biol. 1990 May 20;213(2):215-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/2342104 2342104]
| + | |
| - | [[Category: Homo sapiens]]
| + | |
| - | [[Category: Single protein]]
| + | |
| - | [[Category: Boxer, S G.]]
| + | |
| - | [[Category: Hendrickson, W A.]]
| + | |
| - | [[Category: Hubbard, S R.]]
| + | |
| - | [[Category: Lambright, D G.]]
| + | |
| - | [[Category: Oxygen transport]]
| + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:34:08 2008''
| + | |
