2np1

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (01:14, 21 November 2024) (edit) (undo)
 
(11 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2np1.gif|left|200px]]
 
-
<!--
+
==CRYSTAL STRUCTURE OF NITROPHORIN 1 FROM RHODNIUS PROLIXUS==
-
The line below this paragraph, containing "STRUCTURE_2np1", creates the "Structure Box" on the page.
+
<StructureSection load='2np1' size='340' side='right'caption='[[2np1]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2np1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodnius_prolixus Rhodnius prolixus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NP1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NP1 FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2HP:DIHYDROGENPHOSPHATE+ION'>2HP</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=NH4:AMMONIUM+ION'>NH4</scene></td></tr>
-
{{STRUCTURE_2np1| PDB=2np1 | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2np1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2np1 OCA], [https://pdbe.org/2np1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2np1 RCSB], [https://www.ebi.ac.uk/pdbsum/2np1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2np1 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/NP1_RHOPR NP1_RHOPR] Heme-based protein that deliver nitric oxide gas (NO) to the victim while feeding, resulting in vasodilation and inhibition of platelet aggregation. Also bind tightly to histamine, which is released by the host to induce wound healing.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/np/2np1_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2np1 ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The nitrophorins are heme-based proteins from the salivary glands of the blood-sucking insect Rhodnius prolixus that deliver nitric oxide gas (NO) to the victim while feeding, resulting in vasodilation and inhibition of platelet aggregation. The nitrophorins also bind tightly to histamine, which is released by the host to induce wound healing. Here we present three crystal structures of nitrophorin 1 (NP1): bound to cyanide, which binds in a manner similar to NO (2.3 A resolution); bound to histamine (2.0 A resolution); and bound to what appears to be NH3 from the crystallization solution (2.0 A resolution). The NP1 structures reveal heme to be sandwiched between strands of a lipocalin-like beta-barrel, and in an arrangement unlike any other gas-transport protein discovered to date. The heme is six-coordinate with a histidine (His 59) on the proximal side, and ligand in a spacious pocket on the distal side. The structures confirm that NO and histamine compete for the same binding pocket and become buried on binding. The dissociation constant for histamine binding was found to be 19 nM, approximately 100-fold lower than that for NO.
-
'''CRYSTAL STRUCTURE OF NITROPHORIN 1 FROM RHODNIUS PROLIXUS'''
+
Crystal structures of a nitric oxide transport protein from a blood-sucking insect.,Weichsel A, Andersen JF, Champagne DE, Walker FA, Montfort WR Nat Struct Biol. 1998 Apr;5(4):304-9. PMID:9546222<ref>PMID:9546222</ref>
-
 
+
-
 
+
-
==Overview==
+
-
The nitrophorins are heme-based proteins from the salivary glands of the blood-sucking insect Rhodnius prolixus that deliver nitric oxide gas (NO) to the victim while feeding, resulting in vasodilation and inhibition of platelet aggregation. The nitrophorins also bind tightly to histamine, which is released by the host to induce wound healing. Here we present three crystal structures of nitrophorin 1 (NP1): bound to cyanide, which binds in a manner similar to NO (2.3 A resolution); bound to histamine (2.0 A resolution); and bound to what appears to be NH3 from the crystallization solution (2.0 A resolution). The NP1 structures reveal heme to be sandwiched between strands of a lipocalin-like beta-barrel, and in an arrangement unlike any other gas-transport protein discovered to date. The heme is six-coordinate with a histidine (His 59) on the proximal side, and ligand in a spacious pocket on the distal side. The structures confirm that NO and histamine compete for the same binding pocket and become buried on binding. The dissociation constant for histamine binding was found to be 19 nM, approximately 100-fold lower than that for NO.
+
-
==About this Structure==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
2NP1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rhodnius_prolixus Rhodnius prolixus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NP1 OCA].
+
</div>
 +
<div class="pdbe-citations 2np1" style="background-color:#fffaf0;"></div>
-
==Reference==
+
==See Also==
-
Crystal structures of a nitric oxide transport protein from a blood-sucking insect., Weichsel A, Andersen JF, Champagne DE, Walker FA, Montfort WR, Nat Struct Biol. 1998 Apr;5(4):304-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9546222 9546222]
+
*[[Nitrophorin|Nitrophorin]]
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
[[Category: Rhodnius prolixus]]
[[Category: Rhodnius prolixus]]
-
[[Category: Single protein]]
+
[[Category: Montfort WR]]
-
[[Category: Montfort, W R.]]
+
[[Category: Weichsel A]]
-
[[Category: Weichsel, A.]]
+
-
[[Category: Antihistamine]]
+
-
[[Category: Ferric heme]]
+
-
[[Category: Lipocalin]]
+
-
[[Category: Nitric oxide transport]]
+
-
[[Category: Vasodilator]]
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:43:43 2008''
+

Current revision

CRYSTAL STRUCTURE OF NITROPHORIN 1 FROM RHODNIUS PROLIXUS

PDB ID 2np1

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2np1"
Personal tools