1acc

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /> <applet load="1acc" size="450" color="white" frame="true" align="right" spinBox="true" caption="1acc, resolution 2.1&Aring;" /> '''ANTHRAX PROTECTIVE A...)
Current revision (06:29, 7 February 2024) (edit) (undo)
 
(19 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1acc.gif|left|200px]]<br />
 
-
<applet load="1acc" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1acc, resolution 2.1&Aring;" />
 
-
'''ANTHRAX PROTECTIVE ANTIGEN'''<br />
 
-
==Overview==
+
==ANTHRAX PROTECTIVE ANTIGEN==
-
Protective antigen (PA) is the central component of the three-part protein, toxin secreted by Bacillus anthracis, the organism responsible for, anthrax. After proteolytic activation on the host cell surface, PA forms a, membrane-inserting heptamer that translocates the toxic enzymes, oedema, factor and lethal factor, into the cytosol. PA, which has a relative, molecular mass of 83,000 (M(r) 83K), can also translocate heterologous, proteins, and is being evaluated for use as a general protein delivery, system. Here we report the crystal structure of monomeric PA at 2.1 A, resolution and the water-soluble heptamer at 4.5 A resolution. The monomer, is organized mainly into antiparallel beta-sheets and has four domains: an, amino-terminal domain (domain 1) containing two calcium ions and the, cleavage site for activating proteases; a heptamerization domain (domain, 2) containing a large flexible loop implicated in membrane insertion; a, small domain of unknown function (domain 3); and a carboxy-terminal, receptor-binding domain (domain 4). Removal of a 20K amino-terminal, fragment from domain 1 allows the assembly of the heptamer, a ring-shaped, structure with a negatively charged lumen, and exposes a large hydrophobic, surface for binding the toxic enzymes. We propose a model of pH-dependent, membrane insertion involving the formation of a porin-like, membrane-spanning beta-barrel.
+
<StructureSection load='1acc' size='340' side='right'caption='[[1acc]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1acc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. The April 2002 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Anthrax Toxin'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2002_4 10.2210/rcsb_pdb/mom_2002_4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ACC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ACC FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1acc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1acc OCA], [https://pdbe.org/1acc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1acc RCSB], [https://www.ebi.ac.uk/pdbsum/1acc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1acc ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/PAG_BACAN PAG_BACAN] One of the three proteins composing the anthrax toxin, the agent which infects many mammalian species and that may cause death. PA binds to a receptor (ATR) in sensitive eukaryotic cells, thereby facilitating the translocation of the enzymatic toxin components, edema factor and lethal factor, across the target cell membrane. PA associated with LF causes death when injected, PA associated with EF produces edema. PA induces immunity to infection with anthrax.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ac/1acc_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1acc ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1ACC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. The following page contains interesting information on the relation of 1ACC with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb28_1.html Anthrax Toxin]]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ACC OCA].
+
*[[Anthrax protective antigen|Anthrax protective antigen]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
Crystal structure of the anthrax toxin protective antigen., Petosa C, Collier RJ, Klimpel KR, Leppla SH, Liddington RC, Nature. 1997 Feb 27;385(6619):833-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9039918 9039918]
+
[[Category: Anthrax Toxin]]
[[Category: Anthrax Toxin]]
[[Category: Bacillus anthracis]]
[[Category: Bacillus anthracis]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Liddington, R.C.]]
+
[[Category: RCSB PDB Molecule of the Month]]
-
[[Category: Petosa, C.]]
+
[[Category: Liddington RC]]
-
[[Category: CA]]
+
[[Category: Petosa C]]
-
[[Category: calcium-binding]]
+
-
[[Category: toxin]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 08:56:46 2007''
+

Current revision

ANTHRAX PROTECTIVE ANTIGEN

PDB ID 1acc

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1acc"
Personal tools