2nzo

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[[Image:2nzo.gif|left|200px]]
 
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==Crystal structure of a secretion chaperone CsaA from Bacillus subtilis in the space group P 32 2 1==
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The line below this paragraph, containing "STRUCTURE_2nzo", creates the "Structure Box" on the page.
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<StructureSection load='2nzo' size='340' side='right'caption='[[2nzo]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2nzo]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NZO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NZO FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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{{STRUCTURE_2nzo| PDB=2nzo | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2nzo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nzo OCA], [https://pdbe.org/2nzo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2nzo RCSB], [https://www.ebi.ac.uk/pdbsum/2nzo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2nzo ProSAT]</span></td></tr>
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</table>
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'''Crystal structure of a secretion chaperone CsaA from Bacillus subtilis in the space group P 32 2 1'''
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== Function ==
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[https://www.uniprot.org/uniprot/CSAA_BACSU CSAA_BACSU] Could serves as a molecular chaperone for exported proteins or alternatively acts by stabilizing the SecA protein.
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nz/2nzo_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2nzo ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
Bacillus subtilis CsaA (BsCsaA) has been proposed to act as a protein-secretion chaperone in the Sec-dependent translocation pathway, possibly compensating for the lack of SecB in the Gram-positive eubacterium Bacillus subtilis. This paper presents the cloning, purification, crystallization and structures of BsCsaA in two space groups (P42(1)2 and P3(2)21) solved and refined to resolutions of 1.9 and 2.0 A, respectively. These structures complement the previously available crystal structure of CsaA from the Gram-negative eubacterium Thermus thermophilus (TtCsaA) and provide a direct structural basis for the interpretation of previously available biochemical data on BsCsaA. The sequence and structure of the proposed substrate-binding pocket are analyzed and discussed. A comparison with the TtCsaA structure reveals a different pattern of electrostatic potential in the vicinity of the binding site, which overlaps with a region of high sequence variability. In addition, the dimerization interface of this homodimeric protein is analyzed and discussed.
Bacillus subtilis CsaA (BsCsaA) has been proposed to act as a protein-secretion chaperone in the Sec-dependent translocation pathway, possibly compensating for the lack of SecB in the Gram-positive eubacterium Bacillus subtilis. This paper presents the cloning, purification, crystallization and structures of BsCsaA in two space groups (P42(1)2 and P3(2)21) solved and refined to resolutions of 1.9 and 2.0 A, respectively. These structures complement the previously available crystal structure of CsaA from the Gram-negative eubacterium Thermus thermophilus (TtCsaA) and provide a direct structural basis for the interpretation of previously available biochemical data on BsCsaA. The sequence and structure of the proposed substrate-binding pocket are analyzed and discussed. A comparison with the TtCsaA structure reveals a different pattern of electrostatic potential in the vicinity of the binding site, which overlaps with a region of high sequence variability. In addition, the dimerization interface of this homodimeric protein is analyzed and discussed.
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==About this Structure==
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Crystallographic analysis of Bacillus subtilis CsaA.,Shapova YA, Paetzel M Acta Crystallogr D Biol Crystallogr. 2007 Apr;63(Pt 4):478-85. Epub 2007, Mar 16. PMID:17372352<ref>PMID:17372352</ref>
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2NZO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NZO OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystallographic analysis of Bacillus subtilis CsaA., Shapova YA, Paetzel M, Acta Crystallogr D Biol Crystallogr. 2007 Apr;63(Pt 4):478-85. Epub 2007, Mar 16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17372352 17372352]
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</div>
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<div class="pdbe-citations 2nzo" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Paetzel, M.]]
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[[Category: Paetzel M]]
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[[Category: Shapova, Y A.]]
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[[Category: Shapova YA]]
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[[Category: Beta barrel]]
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[[Category: Homodimer]]
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[[Category: Ob fold]]
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[[Category: Oligonucleotide/oligosaccharide binding fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 10:07:18 2008''
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Current revision

Crystal structure of a secretion chaperone CsaA from Bacillus subtilis in the space group P 32 2 1

PDB ID 2nzo

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