2nzy

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[[Image:2nzy.gif|left|200px]]
 
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==Crystal Structure of alpha1,3-Fucosyltransferase with GDP-fucose==
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The line below this paragraph, containing "STRUCTURE_2nzy", creates the "Structure Box" on the page.
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<StructureSection load='2nzy' size='340' side='right'caption='[[2nzy]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2nzy]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicobacter_pylori Helicobacter pylori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NZY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NZY FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_2nzy| PDB=2nzy | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2nzy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nzy OCA], [https://pdbe.org/2nzy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2nzy RCSB], [https://www.ebi.ac.uk/pdbsum/2nzy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2nzy ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FUCT_HELPX FUCT_HELPX] Involved in the biosynthesis of the Lewis X (LeX) trisaccharide of the lipopolysaccharide (LPS) O-antigen. Catalyzes the addition of fucose in alpha 1-3 linkage to Gal-beta-1-4-GlcNAc-beta-O-R (LacNAc-R) type II acceptor.<ref>PMID:12676935</ref> <ref>PMID:16150700</ref> <ref>PMID:16800635</ref> <ref>PMID:17251184</ref> <ref>PMID:9261149</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nz/2nzy_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2nzy ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Helicobacter pylori alpha1,3-fucosyltransferase (FucT) is involved in catalysis to produce the Lewis x trisaccharide, the major component of the bacteria's lipopolysaccharides, which has been suggested to mimic the surface sugars in gastric epithelium to escape host immune surveillance. We report here three x-ray crystal structures of FucT, including the FucT.GDP-fucose and FucT.GDP complexes. The protein structure is typical of the glycosyltransferase-B family despite little sequence homology. We identified a number of catalytically important residues, including Glu-95, which serves as the general base, and Glu-249, which stabilizes the developing oxonium ion during catalysis. The residues Arg-195, Tyr-246, Glu-249, and Lys-250 serve to interact with the donor substrate, GDP-fucose. Variations in the protein and ligand conformations, as well as a possible FucT dimer, were also observed. We propose a catalytic mechanism and a model of polysaccharide binding not only to explain the observed variations in H. pylori lipopolysaccharides, but also to facilitate the development of potent inhibitors.
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'''Crystal Structure of alpha1,3-Fucosyltransferase with GDP-fucose'''
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Structure and mechanism of Helicobacter pylori fucosyltransferase. A basis for lipopolysaccharide variation and inhibitor design.,Sun HY, Lin SW, Ko TP, Pan JF, Liu CL, Lin CN, Wang AH, Lin CH J Biol Chem. 2007 Mar 30;282(13):9973-82. Epub 2007 Jan 24. PMID:17251184<ref>PMID:17251184</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==About this Structure==
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</div>
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2NZY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Helicobacter_pylori Helicobacter pylori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NZY OCA].
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<div class="pdbe-citations 2nzy" style="background-color:#fffaf0;"></div>
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[[Category: 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Helicobacter pylori]]
[[Category: Helicobacter pylori]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Ko, T P.]]
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[[Category: Ko TP]]
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[[Category: Sun, H Y.]]
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[[Category: Sun HY]]
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[[Category: Alpha1,3-fucosyltransferase]]
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[[Category: Fucosyltransferase]]
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[[Category: Fuct]]
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[[Category: Gt 10]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 10:07:53 2008''
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Current revision

Crystal Structure of alpha1,3-Fucosyltransferase with GDP-fucose

PDB ID 2nzy

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