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1efa

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(New page: 200px<br /> <applet load="1efa" size="450" color="white" frame="true" align="right" spinBox="true" caption="1efa, resolution 2.60&Aring;" /> '''CRYSTAL STRUCTURE O...)
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[[Image:1efa.gif|left|200px]]<br />
 
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<applet load="1efa" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1efa, resolution 2.60&Aring;" />
 
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'''CRYSTAL STRUCTURE OF THE LAC REPRESSOR DIMER BOUND TO OPERATOR AND THE ANTI-INDUCER ONPF'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF THE LAC REPRESSOR DIMER BOUND TO OPERATOR AND THE ANTI-INDUCER ONPF==
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Crystal structures of the Lac repressor, with and without, isopropyithiogalactoside (IPTG), and the repressor bound to operator have, provided a model for how the binding of the inducer reduces the affinity, of the repressor for the operator. However, because of the low resolution, of the operator-bound structure (4.8 A), the model for the allosteric, transition was presented in terms of structural elements rather than in, terms of side chain interactions. Here we have constructed a dimeric Lac, repressor and determined its structure at 2.6 A resolution in complex with, a symmetric operator and the anti-inducer orthonitrophenylfucoside (ONPF)., The structure enables the induced (IPTG-bound) and repressed, (operator-bound) conformations of the repressor to be compared in atomic, detail. An extensive network of interactions between the DNA-binding and, core domains of the repressor suggests a possible mechanism for the, allosteric transition.
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<StructureSection load='1efa' size='340' side='right'caption='[[1efa]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1efa]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. The March 2003 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''lac Repressor'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2003_3 10.2210/rcsb_pdb/mom_2003_3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EFA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EFA FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NPF:ORTHONITROPHENYL-BETA-D-FUCOPYRANOSIDE'>NPF</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1efa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1efa OCA], [https://pdbe.org/1efa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1efa RCSB], [https://www.ebi.ac.uk/pdbsum/1efa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1efa ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LACI_ECOLI LACI_ECOLI] Repressor of the lactose operon. Binds allolactose as an inducer.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ef/1efa_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1efa ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1EFA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with NPF as [http://en.wikipedia.org/wiki/ligand ligand]. The following page contains interesting information on the relation of 1EFA with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb39_1.html lac Repressor]]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EFA OCA].
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*[[Lac repressor|Lac repressor]]
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__TOC__
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==Reference==
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</StructureSection>
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A closer view of the conformation of the Lac repressor bound to operator., Bell CE, Lewis M, Nat Struct Biol. 2000 Mar;7(3):209-14. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10700279 10700279]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: lac Repressor]]
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[[Category: RCSB PDB Molecule of the Month]]
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[[Category: Bell, C.E.]]
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[[Category: Lac Repressor]]
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[[Category: Lewis, M.]]
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[[Category: Bell CE]]
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[[Category: NPF]]
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[[Category: Lewis M]]
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[[Category: gene regulation]]
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[[Category: helix-turn-helix]]
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[[Category: molecular switch]]
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[[Category: protein-dna complex]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 08:59:21 2007''
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Current revision

CRYSTAL STRUCTURE OF THE LAC REPRESSOR DIMER BOUND TO OPERATOR AND THE ANTI-INDUCER ONPF

PDB ID 1efa

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