1ffk

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(New page: 200px<br /> <applet load="1ffk" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ffk, resolution 2.4&Aring;" /> '''CRYSTAL STRUCTURE OF...)
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[[Image:1ffk.gif|left|200px]]<br />
 
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<applet load="1ffk" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ffk, resolution 2.4&Aring;" />
 
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'''CRYSTAL STRUCTURE OF THE LARGE RIBOSOMAL SUBUNIT FROM HALOARCULA MARISMORTUI AT 2.4 ANGSTROM RESOLUTION'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF THE LARGE RIBOSOMAL SUBUNIT FROM HALOARCULA MARISMORTUI AT 2.4 ANGSTROM RESOLUTION==
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The large ribosomal subunit catalyzes peptide bond formation and binds, initiation, termination, and elongation factors. We have determined the, crystal structure of the large ribosomal subunit from Haloarcula, marismortui at 2.4 angstrom resolution, and it includes 2833 of the, subunit's 3045 nucleotides and 27 of its 31 proteins. The domains of its, RNAs all have irregular shapes and fit together in the ribosome like the, pieces of a three-dimensional jigsaw puzzle to form a large, monolithic, structure. Proteins are abundant everywhere on its surface except in the, active site where peptide bond formation occurs and where it contacts the, small subunit. Most of the proteins stabilize the structure by interacting, with several RNA domains, often using idiosyncratically folded extensions, that reach into the subunit's interior.
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<StructureSection load='1ffk' size='340' side='right'caption='[[1ffk]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ffk]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Haloarcula_marismortui Haloarcula marismortui]. The October 2000 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Ribosome'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2000_10 10.2210/rcsb_pdb/mom_2000_10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FFK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FFK FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ffk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ffk OCA], [https://pdbe.org/1ffk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ffk RCSB], [https://www.ebi.ac.uk/pdbsum/1ffk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ffk ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RL2_HALMA RL2_HALMA] One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome (By similarity).[HAMAP-Rule:MF_01320_A]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ff/1ffk_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ffk ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1FFK is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Haloarcula_marismortui Haloarcula marismortui] with CD, K and MG as [http://en.wikipedia.org/wiki/ligands ligands]. The following page contains interesting information on the relation of 1FFK with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb10_1.html Ribosome]]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FFK OCA].
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*[[Large Ribosomal Subunit of Haloarcula|Large Ribosomal Subunit of Haloarcula]]
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*[[Ribosome|Ribosome]]
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==Reference==
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*[[Ribosome 3D structures|Ribosome 3D structures]]
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The complete atomic structure of the large ribosomal subunit at 2.4 A resolution., Ban N, Nissen P, Hansen J, Moore PB, Steitz TA, Science. 2000 Aug 11;289(5481):905-20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10937989 10937989]
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__TOC__
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</StructureSection>
[[Category: Haloarcula marismortui]]
[[Category: Haloarcula marismortui]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: RCSB PDB Molecule of the Month]]
[[Category: Ribosome]]
[[Category: Ribosome]]
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[[Category: Ban, N.]]
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[[Category: Ban N]]
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[[Category: Hansen, J.]]
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[[Category: Hansen J]]
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[[Category: Moore, P.B.]]
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[[Category: Moore PB]]
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[[Category: Nissen, P.]]
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[[Category: Nissen P]]
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[[Category: Steitz, T.A.]]
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[[Category: Steitz TA]]
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[[Category: CD]]
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[[Category: K]]
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[[Category: MG]]
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[[Category: protein-protein]]
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[[Category: protein-rna]]
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[[Category: ribosome assembly]]
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[[Category: rna-rna]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:00:06 2007''
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Current revision

CRYSTAL STRUCTURE OF THE LARGE RIBOSOMAL SUBUNIT FROM HALOARCULA MARISMORTUI AT 2.4 ANGSTROM RESOLUTION

PDB ID 1ffk

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