2o3x

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[[Image:2o3x.gif|left|200px]]
 
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==Crystal Structure of the Prokaryotic Ribosomal Decoding Site Complexed with Paromamine Derivative NB30==
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The line below this paragraph, containing "STRUCTURE_2o3x", creates the "Structure Box" on the page.
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<StructureSection load='2o3x' size='340' side='right'caption='[[2o3x]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2o3x]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O3X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2O3X FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=N30:(1R,2R,3S,4R,6S)-4,6-DIAMINO-2-[(5-AMINO-5-DEOXY-BETA-D-RIBOFURANOSYL)OXY]-3-HYDROXYCYCLOHEXYL+2-AMINO-2-DEOXY-ALPHA-D-GLUCOPYRANOSIDE'>N30</scene></td></tr>
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{{STRUCTURE_2o3x| PDB=2o3x | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2o3x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o3x OCA], [https://pdbe.org/2o3x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2o3x RCSB], [https://www.ebi.ac.uk/pdbsum/2o3x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2o3x ProSAT]</span></td></tr>
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</table>
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'''Crystal Structure of the Prokaryotic Ribosomal Decoding Site Complexed with Paromamine Derivative NB30'''
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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==Overview==
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The lack of absolute prokaryotic selectivity of natural antibiotics is widespread and is a significant clinical problem. The use of this disadvantage of aminoglycoside antibiotics for the possible treatment of human genetic diseases is extremely challenging. Here, we have used a combination of biochemical and structural analysis to compare and contrast the molecular mechanisms of action and the structure-activity relationships of a new synthetic aminoglycoside, NB33, and a structurally similar natural aminoglycoside apramycin. The data presented herein demonstrate the general molecular principles that determine the decreased selectivity of apramycin for the prokaryotic decoding site, and the increased selectivity of NB33 for the eukaryotic decoding site. These results are therefore extremely beneficial for further research on both the design of new aminoglycoside-based antibiotics with diminished deleterious effects on humans, as well as the design of new aminoglycoside-based structures that selectively target the eukaryotic ribosome.
The lack of absolute prokaryotic selectivity of natural antibiotics is widespread and is a significant clinical problem. The use of this disadvantage of aminoglycoside antibiotics for the possible treatment of human genetic diseases is extremely challenging. Here, we have used a combination of biochemical and structural analysis to compare and contrast the molecular mechanisms of action and the structure-activity relationships of a new synthetic aminoglycoside, NB33, and a structurally similar natural aminoglycoside apramycin. The data presented herein demonstrate the general molecular principles that determine the decreased selectivity of apramycin for the prokaryotic decoding site, and the increased selectivity of NB33 for the eukaryotic decoding site. These results are therefore extremely beneficial for further research on both the design of new aminoglycoside-based antibiotics with diminished deleterious effects on humans, as well as the design of new aminoglycoside-based structures that selectively target the eukaryotic ribosome.
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==About this Structure==
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Differential selectivity of natural and synthetic aminoglycosides towards the eukaryotic and prokaryotic decoding A sites.,Kondo J, Hainrichson M, Nudelman I, Shallom-Shezifi D, Barbieri CM, Pilch DS, Westhof E, Baasov T Chembiochem. 2007 Sep 24;8(14):1700-9. PMID:17705310<ref>PMID:17705310</ref>
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2O3X is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O3X OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Differential selectivity of natural and synthetic aminoglycosides towards the eukaryotic and prokaryotic decoding A sites., Kondo J, Hainrichson M, Nudelman I, Shallom-Shezifi D, Barbieri CM, Pilch DS, Westhof E, Baasov T, Chembiochem. 2007 Sep 24;8(14):1700-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17705310 17705310]
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</div>
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[[Category: Single protein]]
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<div class="pdbe-citations 2o3x" style="background-color:#fffaf0;"></div>
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[[Category: Baasov, T.]]
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== References ==
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[[Category: Hainrichson, M.]]
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<references/>
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[[Category: Kondo, J.]]
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__TOC__
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[[Category: Nudelman, I.]]
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</StructureSection>
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[[Category: Shallom-Shezifi, D.]]
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[[Category: Large Structures]]
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[[Category: Westhof, E.]]
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[[Category: Baasov T]]
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[[Category: Aminoglycoside]]
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[[Category: Hainrichson M]]
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[[Category: Antibiotic]]
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[[Category: Kondo J]]
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[[Category: Decoding site]]
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[[Category: Nudelman I]]
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[[Category: Prokaryote]]
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[[Category: Shallom-Shezifi D]]
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[[Category: Ribosome]]
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[[Category: Westhof E]]
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[[Category: Rna]]
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[[Category: Stop codon readthrough]]
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[[Category: Translation inhibition]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 10:17:52 2008''
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Crystal Structure of the Prokaryotic Ribosomal Decoding Site Complexed with Paromamine Derivative NB30

PDB ID 2o3x

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